Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-280 of Human Wnt11 (NP_004617).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates for WB, HeLa cells for IF and Lung SCC for IHC-P.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/100 - 1/250.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity

    Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas.
  • Sequence similarities

    Belongs to the Wnt family.
  • Post-translational
    modifications

    Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • HWNT11 antibody
    • Protein Wnt-11 antibody
    • Wingless-related MMTV integration site 11 antibody
    • Wingless-type MMTV integration site family, member 1 antibody
    • Wingless-type MMTV integration site family, member 11 antibody
    • Wingless-type MMTV integration site family, member 11B antibody
    • wnt-11 antibody
    • wnt11 antibody
    • WNT11_HUMAN antibody
    • wnt11b antibody
    see all

Images

  • Anti-Wnt11 antibody (ab96730) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded lung SCC, using ab96730 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96730 at 1/200 dilution. Lower image merged with DNA probe.

References

This product has been referenced in:

  • Roettger A  et al. Inhibitory Effect of 1,8-Cineol on ß-Catenin Regulation, WNT11 Expression, and Cellular Progression in HNSCC. Front Oncol 7:92 (2017). Read more (PubMed: 28589081) »
  • Keats EC  et al. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells 32:1649-60 (2014). Read more (PubMed: 24496952) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult ovary and uterus)
Permeabilization
Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification
Adult ovary and uterus
Blocking step
3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 21 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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