Recombinant Anti-Wnt16 antibody [EPR22889-129] (ab269278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22889-129] to Wnt16
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt16 antibody [EPR22889-129]
See all Wnt16 primary antibodies -
Description
Rabbit monoclonal [EPR22889-129] to Wnt16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: IMR-90 and HEK-293 whole cell lysates. IP: HEK-293 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22889-129 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa). |
IP
1/30. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Tissue specificity
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 51384 Human
- Omim: 606267 Human
- SwissProt: Q9UBV4 Human
- Unigene: 272375 Human
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Alternative names
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
see all
Images
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All lanes : Anti-Wnt16 antibody [EPR22889-129] (ab269278) at 1/1000 dilution
Lane 1 : IMR-90 (human lung fibroblast) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile observed is consistent with what has been described in the literature, the band of 45 KDa represents the full length Wnt16, the band of 32 KDa represents the degraded form of Wnt16. (PMID:26254351, 17200136).
Negative control: U-2 OS (PMID: 25999350).
This blot was developed using a higher sensitivity ECL substrate.Blocking/Diluting Buffer: 5% NFDM/TBST
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Wnt16 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate using ab269278 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab269278 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used as the secondary anyibody at 1/5000 dilution.
Lane 1: HEK-293 whole cell lysate (input)
Lane 2: ab269278 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269278 in HEK-293 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 mintues
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab269278 has not yet been referenced specifically in any publications.