Product nameAnti-Wnt16 antibody [EPR22889-129]
See all Wnt16 primary antibodies
DescriptionRabbit monoclonal [EPR22889-129] to Wnt16
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human Wnt16 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UBV4
- WB: IMR-90 and HEK-293 whole cell lysates. IP: HEK-293 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab269278 in the following tested applications.
|WB||1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Tissue specificityIsoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
All lanes : Anti-Wnt16 antibody [EPR22889-129] (ab269278) at 1/1000 dilution
Lane 1 : IMR-90 (human lung fibroblast) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
The expression profile observed is consistent with what has been described in the literature, the band of 45 KDa represents the full length Wnt16, the band of 32 KDa represents the degraded form of Wnt16. (PMID:26254351, 17200136).
Negative control: U-2 OS (PMID: 25999350).
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Diluting Buffer: 5% NFDM/TBST
Wnt16 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate using ab269278 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab269278 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used as the secondary anyibody at 1/5000 dilution.
Lane 1: HEK-293 whole cell lysate (input)
Lane 2: ab269278 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269278 in HEK-293 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 mintues
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269278 has not yet been referenced specifically in any publications.