Recombinant Anti-Wnt16 antibody [EPR6064] (ab109437)

Thank you for this data.

After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came up which identities which while not at 85% were near that threshold. Most of these results would show bands that where very different from the expected size for Wnt16 (41kDa). However AAB91426.1 aspartate aminotransferase precursor, which shared 78% identity, did show an expected band near 47kDa.

This issue is very concerning to us and we are bringing this to the attention of our lab. So that I may provide them with the information they most need to investigate this, could you please provide us with details of the western blot experiments including sample preparation? It would also be very helpful to us if you could send is the size of these bands or an uncut blot image.


I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us.

I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.

Would you be able to provide details regarding your sample, protocol and troubleshooting step that have been taken. Images are very helpful as well. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well. Could you also provide the Abcam order confirmation number or the PO and date of purchase for this product?


I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence is proprietary (commercially sensitive and confidential). Therefore, please do not share or publish this information. It is strictly for your own use. I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

DISCOUNT CODE: 00000

Expiration date:04. January 2013

Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to your customer testing ab109437 in IHC-Fr.

This code will give your customer the value ofab109437 off a future purchase before the expiration date.

To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.