RecombinantRabMAb

Recombinant Anti-Wnt16 antibody [EPR6064] (ab109437)

Reviews (2)Q&A (4)References (7)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6064] to Wnt16
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Wnt16 antibody [EPR6064]
    See all Wnt16 primary antibodies

  • Description

    Rabbit monoclonal [EPR6064] to Wnt16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Wnt16 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, LnCaP, HepG2, 293T, Mouse brain, and Rat brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.05 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6064

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

    • Tissue specificity

      Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.

    • Sequence similarities

      Belongs to the Wnt family.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • Protein Wnt 16 precursor antibody
        • Protein Wnt-16 antibody
        • Wingless type MMTV integration site family member 16 antibody
        • WNT16 antibody
        • WNT16_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution (Purified)

        Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
        Lane 2 : Mouse brain lysates
        Lane 3 : Rat brain lysates

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 41 kDa
        Observed band size: 41 kDa

      • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

        Lane 1 : LnCaP cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 41 kDa

      • Other - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Other - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References (7)

      Publishing research using ab109437? Please let us know so that we can cite the reference in this datasheet.

      ab109437 has been referenced in 7 publications.

      • Guo G  et al. Hsa_circ_0000479 as a Novel Diagnostic Biomarker of Systemic Lupus Erythematosus. Front Immunol 10:2281 (2019). PubMed: 31608065
      • Ding SM  et al. The role of cancer-associated fibroblast MRC-5 in pancreatic cancer. J Cancer 9:614-628 (2018). WB ; Human . PubMed: 29483967
      • Hildebrandt S  et al. Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo. Sci Rep 8:8711 (2018). WB ; Mouse . PubMed: 29880826
      • Spinelli C  et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643
      • Munguía-Reyes A  et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
      • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
      • Andres-Mateos E  et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other species . PubMed: 23155423

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-6 of 6 Abreviews or Q&A

      IHC - Wholemount abreview for Anti-Wnt16 antibody [EPR6064]

       Inconclusive
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
      Specification
      Wholemount 3-5 days post fertilisation
      Read More

      Dr. Chrissy Hammond

      Verified customer

      Submitted Jul 23 2013

      Immunohistochemistry (Frozen sections) abreview for Anti-Wnt16 antibody [EPR6064]

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Liver, Spleen)
      Specification
      Liver, Spleen
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% TritonX
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 31 2012

      Question

      yes, sure I am happy to provide you with evidence showing that this antibody is not a specific antibody. attached please find the AI file. actually this antibody has wasted a lot of my samples and also time. thanks for your help.

      Read More
      Answer

      Thank you for this data.

      After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came up which identities which while not at 85% were near that threshold. Most of these results would show bands that where very different from the expected size for Wnt16 (41kDa). However AAB91426.1 aspartate aminotransferase precursor, which shared 78% identity, did show an expected band near 47kDa.

      This issue is very concerning to us and we are bringing this to the attention of our lab. So that I may provide them with the information they most need to investigate this, could you please provide us with details of the western blot experiments including sample preparation? It would also be very helpful to us if you could send is the size of these bands or an uncut blot image.


      I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Thanks for your reply, from the results I have got,I think this antibody is not specific to Wnt16 only, because in the samples I got from wnt16 knock out mice it has a huge expression of wnt16 using your wnt16 antibody by WB. but Q-PCR showed no expression of wnt16 at mRNA level. Today I BLAST the sequence you provided , and comes out a lot similar protein which has similar epitope. prohibited.

      Read More
      Answer

      Thank you for contacting us.

      I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.

      Would you be able to provide details regarding your sample, protocol and troubleshooting step that have been taken. Images are very helpful as well. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well. Could you also provide the Abcam order confirmation number or the PO and date of purchase for this product?


      I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      on your website I can't find the immunogen sequence for this antibody :Anti-Wnt16 antibody [EPR6064] (ab109437) Could you please tell me ?

      Read More
      Answer

      Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence is proprietary (commercially sensitive and confidential). Therefore, please do not share or publish this information. It is strictly for your own use. I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

      Read More

      Question

      Dear Supplier,



      I have a customer who has purchased 1 x Anti-Wnt16 antibody [EPR6064] (ab109437) in May 2012. They have not tested it yet. They were wondering if they can get a testing discount approved for this product. They are looking at testing on mouse pancreas and spleen IHC-Fr sections.



      I have explained in detailed how the testing discounts works to the customer. Unfortunately in this case they have purchased product first before notifying Sapphire their intentions.



      This was purchased by Sapphire on our purchased order PO-13970, dated 02/05/12. Lot# is GR52815.



      Customer`s details are:





      Looking forward for your response.





      Kind regards,

      Read More
      Answer

      DISCOUNT CODE: 00000

      Expiration date:04. January 2013

      Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to your customer testing ab109437 in IHC-Fr.

      This code will give your customer the value ofab109437 off a future purchase before the expiration date.

      To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

      The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

      1) Call to place your order and mention the code to our customer service department;

      2) Include the code in your fax order;

      3) Place your order on the web and enter the promotional code.

      Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com