Recombinant
RabMAb

Recombinant Anti-Wnt16 antibody [EPR6064] (ab109437)

Overview

  • Product name

    Anti-Wnt16 antibody [EPR6064]
    See all Wnt16 primary antibodies
  • Description

    Rabbit monoclonal [EPR6064] to Wnt16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt16 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, LnCaP, HepG2, 293T, Mouse brain, and Rat brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.05 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6064
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Tissue specificity

      Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
    • Sequence similarities

      Belongs to the Wnt family.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Protein Wnt 16 precursor antibody
      • Protein Wnt-16 antibody
      • Wingless type MMTV integration site family member 16 antibody
      • WNT16 antibody
      • WNT16_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution (Purified)

      Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    • All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 5 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
    Specification
    Wholemount 3-5 days post fertilisation

    Dr. Chrissy Hammond

    Verified customer

    Submitted Jul 23 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver, Spleen)
    Specification
    Liver, Spleen
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Answer

    Thank you for this data.

    After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came up which identities which while not at 85% were near that threshold. Most of these results would show bands that where very different from the expected size for Wnt16 (41kDa). However AAB91426.1 aspartate aminotransferase precursor, which shared 78% identity, did show an expected band near 47kDa.

    This issue is very concerning to us and we are bringing this to the attention of our lab. So that I may provide them with the information they most need to investigate this, could you please provide us with details of the western blot experiments including sample preparation? It would also be very helpful to us if you could send is the size of these bands or an uncut blot image.


    I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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    Answer

    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.

    Would you be able to provide details regarding your sample, protocol and troubleshooting step that have been taken. Images are very helpful as well. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well. Could you also provide the Abcam order confirmation number or the PO and date of purchase for this product?


    I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Answer

    Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence is proprietary (commercially sensitive and confidential). Therefore, please do not share or publish this information. It is strictly for your own use. I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

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    Answer

    DISCOUNT CODE: 00000

    Expiration date:04. January 2013

    Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to your customer testing ab109437 in IHC-Fr.

    This code will give your customer the value ofab109437 off a future purchase before the expiration date.

    To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

    1) Call to place your order and mention the code to our customer service department;

    2) Include the code in your fax order;

    3) Place your order on the web and enter the promotional code.

    Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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