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    wnt16-antibody-epr6064-ab109437.pdf

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Stem Cells Signaling Pathways Wnt Secreted
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RecombinantRabMAb

Recombinant Anti-Wnt16 antibody [EPR6064] (ab109437)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (7)

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Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
  • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
  • OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (ab109437)
  • Anti-Wnt16 antibody [EPR6064] (ab109437)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6064] to Wnt16
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Wnt16 antibody [EPR6064]
    See all Wnt16 primary antibodies
  • Description

    Rabbit monoclonal [EPR6064] to Wnt16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, LnCaP, HepG2, 293T, Mouse brain, and Rat brain lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.05 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6064
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Associated products

  • Alternative Versions

    • Anti-Wnt16 antibody [EPR6064] - BSA and Azide free (ab247863)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity

    Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q9UBV4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51384 Human
    • Entrez Gene: 93735 Mouse
    • Entrez Gene: 500047 Rat
    • Omim: 606267 Human
    • SwissProt: Q9UBV4 Human
    • SwissProt: Q9QYS1 Mouse
    • Unigene: 272375 Human
    • Unigene: 137403 Mouse
    • Alternative names

      • Protein Wnt 16 precursor antibody
      • Protein Wnt-16 antibody
      • Wingless type MMTV integration site family member 16 antibody
      • WNT16 antibody
      • WNT16_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
      Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
      All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution (Purified)

      Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
      Lane 2 : Mouse brain lysates
      Lane 3 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
      Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
      All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (ab109437)
      OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (ab109437)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Wnt16 antibody [EPR6064] (ab109437)
      Anti-Wnt16 antibody [EPR6064] (ab109437)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab109437? Please let us know so that we can cite the reference in this datasheet.

    ab109437 has been referenced in 7 publications.

    • Guo G  et al. Hsa_circ_0000479 as a Novel Diagnostic Biomarker of Systemic Lupus Erythematosus. Front Immunol 10:2281 (2019). PubMed: 31608065
    • Ding SM  et al. The role of cancer-associated fibroblast MRC-5 in pancreatic cancer. J Cancer 9:614-628 (2018). WB ; Human . PubMed: 29483967
    • Hildebrandt S  et al. Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo. Sci Rep 8:8711 (2018). WB ; Mouse . PubMed: 29880826
    • Spinelli C  et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643
    • Munguía-Reyes A  et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
    • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
    • Andres-Mateos E  et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other species . PubMed: 23155423

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Wnt16 antibody [EPR6064]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
    Specification
    Wholemount 3-5 days post fertilisation
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Chrissy Hammond

    Verified customer

    Submitted Jul 23 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Wnt16 antibody [EPR6064]

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver, Spleen)
    Specification
    Liver, Spleen
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Question

    yes, sure I am happy to provide you with evidence showing that this antibody is not a specific antibody. attached please find the AI file. actually this antibody has wasted a lot of my samples and also time. thanks for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 30 2012

    Answer

    Thank you for this data.

    After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came up which identities which while not at 85% were near that threshold. Most of these results would show bands that where very different from the expected size for Wnt16 (41kDa). However AAB91426.1 aspartate aminotransferase precursor, which shared 78% identity, did show an expected band near 47kDa.

    This issue is very concerning to us and we are bringing this to the attention of our lab. So that I may provide them with the information they most need to investigate this, could you please provide us with details of the western blot experiments including sample preparation? It would also be very helpful to us if you could send is the size of these bands or an uncut blot image.


    I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 30 2012

    Question

    Thanks for your reply, from the results I have got,I think this antibody is not specific to Wnt16 only, because in the samples I got from wnt16 knock out mice it has a huge expression of wnt16 using your wnt16 antibody by WB. but Q-PCR showed no expression of wnt16 at mRNA level. Today I BLAST the sequence you provided , and comes out a lot similar protein which has similar epitope. prohibited.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within the last six months, we are happy to provide scientific support and to replace or refund the product should we not be able to help.

    Would you be able to provide details regarding your sample, protocol and troubleshooting step that have been taken. Images are very helpful as well. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well. Could you also provide the Abcam order confirmation number or the PO and date of purchase for this product?


    I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    on your website I can't find the immunogen sequence for this antibody :Anti-Wnt16 antibody [EPR6064] (ab109437) Could you please tell me ?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence is proprietary (commercially sensitive and confidential). Therefore, please do not share or publish this information. It is strictly for your own use. I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    Dear Supplier,



    I have a customer who has purchased 1 x Anti-Wnt16 antibody [EPR6064] (ab109437) in May 2012. They have not tested it yet. They were wondering if they can get a testing discount approved for this product. They are looking at testing on mouse pancreas and spleen IHC-Fr sections.



    I have explained in detailed how the testing discounts works to the customer. Unfortunately in this case they have purchased product first before notifying Sapphire their intentions.



    This was purchased by Sapphire on our purchased order PO-13970, dated 02/05/12. Lot# is GR52815.



    Customer`s details are:





    Looking forward for your response.





    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    DISCOUNT CODE: 00000

    Expiration date:04. January 2013

    Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to your customer testing ab109437 in IHC-Fr.

    This code will give your customer the value ofab109437 off a future purchase before the expiration date.

    To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

    1) Call to place your order and mention the code to our customer service department;

    2) Include the code in your fax order;

    3) Place your order on the web and enter the promotional code.

    Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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