Recombinant
RabMAb

Anti-Wnt16 antibody [EPR6064] (ab109437)

Abreviews (2)Q&A (4)Specific References (4)

Overview

  • Product name
    Anti-Wnt16 antibody [EPR6064]
    See all Wnt16 primary antibodies
  • Description
    Rabbit monoclonal [EPR6064] to Wnt16
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt16 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • LnCaP, HepG2, Raji, and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 6.05 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6064
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Tissue specificity
      Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
    • Sequence similarities
      Belongs to the Wnt family.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
      • Alternative names
        • Protein Wnt 16 precursor antibody
        • Protein Wnt-16 antibody
        • Wingless type MMTV integration site family member 16 antibody
        • WNT16 antibody
        • WNT16_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)
        All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution

        Lane 1 : LnCaP cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 41 kDa

      • Other - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Other - Anti-Wnt16 antibody [EPR6064] (ab109437)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      See all 4 Publications for this product

      Publishing research using ab109437? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      6 reviews or Q&A

      10
      Votes: Highest First

      IHC - Wholemount abreview for Anti-Wnt16 antibody [EPR6064]

       Inconclusive
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      IHC - Wholemount
      Sample
      Zebrafish Embryo (Wholemount 3-5 days post fertilisation)
      Specification
      Wholemount 3-5 days post fertilisation
      Read More
      abreview image
      Username

      Dr. Chrissy Hammond

      Verified customer

      Submitted Jul 23 2013

      Immunohistochemistry (Frozen sections) abreview for Anti-Wnt16 antibody [EPR6064]

       Inconclusive
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Liver, Spleen)
      Specification
      Liver, Spleen
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% TritonX
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Read More
      Username

      Abcam user community

      Verified customer

      Submitted Oct 31 2012

      yes, sure I am happy to provide you with evidence showing that this antibody is not a specific antibody. attached please find the AI file. actually this antibody has wasted a lot of my samples and also time. thanks for your help.

      Thank you for this data.

      After review of this data, I was able to repeat a BLAST of this immunogen. Generally antibodies will not work well with products which have less than 85% homology with the immunogen. I did see that many results came ...

      Read More

      Thanks for your reply, from the results I have got,I think this antibody is not specific to Wnt16 only, because in the samples I got from wnt16 knock out mice it has a huge expression of wnt16 using your wnt16 antibody by WB. but Q-PCR showed no expre...

      Read More

      Thank you for contacting us.

      I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased withi...

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      on your website I can't find the immunogen sequence for this antibody :Anti-Wnt16 antibody [EPR6064] (ab109437) Could you please tell me ?

      Thank you for contacting us and your interest in our products. I am pleased to provide the following immunogen information: xxxxxxxx In order to aid your research we are happy to provide this information to you. However, this immunogen sequence...

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      Dear Supplier,



      I have a customer who has purchased 1 x Anti-Wnt16 antibody [EPR6064] (ab109437) in May 2012. They have not tested it yet. They were wondering if they can get a testing discount approved for this product. They are ...

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      DISCOUNT CODE: 00000

      Expiration date:04. January 2013

      Thank thank you for your inquiry. I am happy to confirm that I am able to make an exception and provide a code after the purchase of this antibody. We are looking for ward to you...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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