Recombinant Anti-Wnt16 antibody [EPR6064] (ab109437)
![Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)](https://www.abcam.com/ps/products/109/ab109437/Images/ab109437-365436-anti-wnt16-antibody-epr6064-westernblot-human-mouse-rat.jpg "Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6064] to Wnt16
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt16 antibody [EPR6064]
See all Wnt16 primary antibodies -
Description
Rabbit monoclonal [EPR6064] to Wnt16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, LnCaP, HepG2, 293T, Mouse brain, and Rat brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.05 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6064 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Tissue specificity
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 51384 Human
- Entrez Gene: 93735 Mouse
- Entrez Gene: 500047 Rat
- Omim: 606267 Human
- SwissProt: Q9UBV4 Human
- SwissProt: Q9QYS1 Mouse
- Unigene: 272375 Human
- Unigene: 137403 Mouse
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Alternative names
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
see all
Images
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Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution (Purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
![Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)](https://www.abcam.com/ps/products/109/ab109437/Images/ab109437-122281-anti-wnt16-antibody-epr6064-western-blot.jpg)
Western blot - Anti-Wnt16 antibody [EPR6064] (ab109437)All lanes : Anti-Wnt16 antibody [EPR6064] (ab109437) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
![OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (ab109437)](https://www.abcam.com/ps/products/109/ab109437/Images/ab109437-122280-anti-wnt16-antibody-epr6064.jpg)
OI-RD Scanning - Anti-Wnt16 antibody [EPR6064] (ab109437) -
![Anti-Wnt16 antibody [EPR6064] (ab109437)](https://www.abcam.com/ps/products/109/ab109437/Images/ab109437-4-benefits-of-recombinant-antibodies.png)
Anti-Wnt16 antibody [EPR6064] (ab109437)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109437 has been referenced in 7 publications.
- Guo G et al. Hsa_circ_0000479 as a Novel Diagnostic Biomarker of Systemic Lupus Erythematosus. Front Immunol 10:2281 (2019). PubMed: 31608065
- Ding SM et al. The role of cancer-associated fibroblast MRC-5 in pancreatic cancer. J Cancer 9:614-628 (2018). WB ; Human . PubMed: 29483967
- Hildebrandt S et al. Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo. Sci Rep 8:8711 (2018). WB ; Mouse . PubMed: 29880826
- Spinelli C et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643
- Munguía-Reyes A et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Andres-Mateos E et al. Impaired skeletal muscle regeneration in the absence of fibrosis during hibernation in 13-lined ground squirrels. PLoS One 7:e48884 (2012). WB ; Other species . PubMed: 23155423