Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt2b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt2b aa 341-391 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    GRGYDTTRVT RVTQCECKFH WCCAVRCKEC RNTVDVHTCK APKKAEWLDQ T


    Database link: Q93097

  • Positive control

    • Rat brain and mouse intestine lysates. Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab203225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
  • Tissue specificity

    Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein Wnt-13 antibody
    • Protein Wnt-2b antibody
    • Wingless type MMTV integration site family, member 13 antibody
    • Wingless type MMTV integration site family, member 2B antibody
    • WNT13 antibody
    • Wnt2b antibody
    • WNT2B_HUMAN antibody
    • XWNT2 antibody
    • XWNT2, Xenopus, homolog of antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Wnt2b using ab203225 at 1/250, followed by secondary detection and DAB staining.

  • All lanes : Anti-Wnt2b antibody (ab203225) at 1/200 dilution

    Lane 1 : rat brain lysate
    Lane 2 : mouse intestine lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 44 kDa

References

ab203225 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203225.
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