Recombinant
RabMAb

Recombinant Anti-Wnt2b antibody [EPR13386] (ab178418)

Overview

  • Product name

    Anti-Wnt2b antibody [EPR13386]
    See all Wnt2b primary antibodies
  • Description

    Rabbit monoclonal [EPR13386] to Wnt2b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt2b aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q93097

  • Positive control

    • WB: Human fetal brain and heart lysates; HepG2 and Jurkat cells and cell lysates. ICC/IF: HT-29 and HepG2 cells. Flow Cyt: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
    • Tissue specificity

      Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
    • Sequence similarities

      Belongs to the Wnt family.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Protein Wnt-13 antibody
      • Protein Wnt-2b antibody
      • Wingless type MMTV integration site family, member 13 antibody
      • Wingless type MMTV integration site family, member 2B antibody
      • WNT13 antibody
      • Wnt2b antibody
      • WNT2B_HUMAN antibody
      • XWNT2 antibody
      • XWNT2, Xenopus, homolog of antibody
      see all

    Images

    • All lanes : Anti-Wnt2b antibody [EPR13386] (ab178418) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • ab178418 staining Wnt2b in HT-29 (human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control: PBS only.

    • Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Wnt2b with ab178418 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Flow Cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Wnt2b with ab178418 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Yes, permeabilization is necessary and was used in our validation of ab178418 in flow cytometry. Secreted proteins such as Wnt2b are synthesized, processed and stored before secretion, these all happen in the cytoplasm. Permeabilisation allows antibodies to have access to the antigen in the cytoplasm of the cell, so this process is necessary in flow cytometry for a secreted protein.

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