RecombinantRabMAb

Recombinant Anti-Wnt2b antibody [EPR13386] (ab178418)

Submit a review Q&A (1)References (2)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13386] to Wnt2b
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Wnt2b antibody [EPR13386]
    See all Wnt2b primary antibodies

  • Description

    Rabbit monoclonal [EPR13386] to Wnt2b

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Wnt2b aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q93097

  • Positive control

    • WB: Human fetal brain and heart lysates; HepG2 and Jurkat cells and cell lysates. ICC/IF: HT-29 and HepG2 cells. Flow Cyt: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab178418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

    • Tissue specificity

      Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.

    • Sequence similarities

      Belongs to the Wnt family.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Protein Wnt-13 antibody
      • Protein Wnt-2b antibody
      • Wingless type MMTV integration site family, member 13 antibody
      • Wingless type MMTV integration site family, member 2B antibody
      • WNT13 antibody
      • Wnt2b antibody
      • WNT2B_HUMAN antibody
      • XWNT2 antibody
      • XWNT2, Xenopus, homolog of antibody
      see all

    Images

    • Western blot - Anti-Wnt2b antibody [EPR13386] (ab178418)
      Western blot - Anti-Wnt2b antibody [EPR13386] (ab178418)
      All lanes : Anti-Wnt2b antibody [EPR13386] (ab178418) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)
      Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)

      ab178418 staining Wnt2b in HT-29 (human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control: PBS only.

    • Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)
      Immunocytochemistry/ Immunofluorescence - Anti-Wnt2b antibody [EPR13386] (ab178418)

      Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Wnt2b with ab178418 at 1/50 dilution (green). DAPI nuclear staining (blue).

    • Flow Cytometry - Anti-Wnt2b antibody [EPR13386] (ab178418)
      Flow Cytometry - Anti-Wnt2b antibody [EPR13386] (ab178418)

      Flow Cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Wnt2b with ab178418 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    • Anti-Wnt2b antibody [EPR13386] (ab178418)
      Anti-Wnt2b antibody [EPR13386] (ab178418)

    References (2)

    Publishing research using ab178418? Please let us know so that we can cite the reference in this datasheet.

    ab178418 has been referenced in 2 publications.

    • Zhang H  et al. Expression profile and clinical significance of Wnt signaling in human gliomas. Oncol Lett 15:610-617 (2018). PubMed: 29387236
    • Sun X  et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). PubMed: 28147310

    Customer reviews and Q&As

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    Question

    Is it necessary for the cells to be permeablized during flow cytometry for this product to work?

    Read More
    Answer

    Yes, permeabilization is necessary and was used in our validation of ab178418 in flow cytometry. Secreted proteins such as Wnt2b are synthesized, processed and stored before secretion, these all happen in the cytoplasm. Permeabilisation allows antibodies to have access to the antigen in the cytoplasm of the cell, so this process is necessary in flow cytometry for a secreted protein.

    Read More

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