Recombinant Anti-Wnt2b antibody [EPR13386] (ab178418)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13386] to Wnt2b
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt2b antibody [EPR13386]
See all Wnt2b primary antibodies -
Description
Rabbit monoclonal [EPR13386] to Wnt2b -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Wnt2b aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q93097 -
Positive control
- WB: Human fetal brain and heart lysates; HepG2 and Jurkat cells and cell lysates. ICC/IF: HT-29 and HepG2 cells. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13386 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/50 - 1/100.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/5000. Predicted molecular weight: 44 kDa. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. -
Tissue specificity
Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7482 Human
- Entrez Gene: 22414 Mouse
- Entrez Gene: 116466 Rat
- Omim: 601968 Human
- SwissProt: Q93097 Human
- SwissProt: O70283 Mouse
- Unigene: 258575 Human
- Unigene: 10740 Mouse
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Alternative names
- Protein Wnt-13 antibody
- Protein Wnt-2b antibody
- Wingless type MMTV integration site family, member 13 antibody
see all
Images
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All lanes : Anti-Wnt2b antibody [EPR13386] (ab178418) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa -
ab178418 staining Wnt2b in HT-29 (human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control: PBS only.
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Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Wnt2b with ab178418 at 1/50 dilution (green). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Wnt2b with ab178418 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab178418 has been referenced in 3 publications.
- Wu CL et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun 12:362 (2021). PubMed: 33441552
- Zhang H et al. Expression profile and clinical significance of Wnt signaling in human gliomas. Oncol Lett 15:610-617 (2018). PubMed: 29387236
- Sun X et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). PubMed: 28147310