Recombinant
RabMAb

Recombinant Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free (ab232036)

Overview

  • Product name

    Anti-Wnt2/IRP antibody [EPR3101(2)] - BSA and Azide free
    See all Wnt2/IRP primary antibodies
  • Description

    Rabbit monoclonal [EPR3101(2)] to Wnt2/IRP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt2/IRP aa 250-350. The exact sequence is proprietary.
    (Peptide available as ab197186)

  • Positive control

    • WB: Mouse brain tissue lysate.
  • General notes

    ab232036 is the carrier-free version of ab109222 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232036 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Wnt2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa).

Can be blocked with ab197186

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity

    Expressed in brain in the thalamus, in fetal and adult lung and in placenta.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Form

    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis.
  • Alternative names

    • Int 1 related protein antibody
    • Int-1-like protein 1 antibody
    • Int-1-related protein antibody
    • INT1L 1 antibody
    • INT1L1 antibody
    • IRP antibody
    • IRP protein antibody
    • ONCOGENE INT1 LIKE 1 antibody
    • Protein Wnt-2 antibody
    • Secreted growth factor antibody
    • Wingless type MMTV integration site family member 2 antibody
    • WNT 2 antibody
    • WNT2 antibody
    • WNT2_HUMAN antibody
    see all

Images

  • Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 40 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109222).

References

ab232036 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232036.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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