Recombinant
RabMAb

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)

Overview

  • Product name
    Anti-Wnt3 and Wnt3a antibody [EPR4920(2)]
  • Description
    Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt3 and Wnt3a aa 150-250 (Cysteine residue). The exact sequence is proprietary. SwissProt: P56703 and P56704
    Database link: P56704

  • Positive control
    • CHO cells transfected with Human Wnt3a.
  • General notes

    This antibody was developed as part of a collaboration with Karl Willert’s lab from the University of California at San Diego.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      Proto-oncogene Wnt-3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Protein Wnt-3a: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
    • Database links

    Images

    • All lanes : Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612) at 1/10000 dilution

      Lane 1 : CHO cell lysate, non-transfected
      Lane 2 : CHO cell lysate, transfected with Human Wnt3a

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    References

    This product has been referenced in:
    • Guo F  et al. The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway. Exp Ther Med 15:4885-4889 (2018). Read more (PubMed: 29805510) »
    • Lippert A  et al. Single-Molecule Imaging of Wnt3A Protein Diffusion on Living Cell Membranes. Biophys J 113:2762-2767 (2017). Read more (PubMed: 29262368) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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