Recombinant Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free (ab240167)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] - BSA and Azide free
See all Wnt3 and Wnt3a primary antibodies -
Description
Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240167 is the carrier-free version of ab172612.
This antibody was developed as part of a collaboration with Karl Willert’s lab from the University of California at San Diego.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4920(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa. |
Target
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Relevance
Proto-oncogene Wnt-3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Protein Wnt-3a: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Database links
- Entrez Gene: 7473 Human
- Entrez Gene: 89780 Human
- SwissProt: P56703 Human
- SwissProt: P56704 Human
Images
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All lanes : Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612) at 1/10000 dilution
Lane 1 : CHO cell lysate, non-transfected
Lane 2 : CHO cell lysate, transfected with Human Wnt3a
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDaThis data was developed using ab172612, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240167 has not yet been referenced specifically in any publications.