Anti-Wnt3 antibody (ab116222)
Key features and details
- Goat polyclonal to Wnt3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3 antibody
See all Wnt3 primary antibodies -
Description
Goat polyclonal to Wnt3 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide:
CGRGHNTRTEKRKEK
, corresponding to C terminal amino acids 315-329 of Human Wnt3. -
Positive control
- WB: Human brain lysate IHC-P: Human adrenal, breast and thyroid tissues
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab116222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa. | |
IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Involvement in disease
Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 374142 Chicken
- Entrez Gene: 7473 Human
- Entrez Gene: 22415 Mouse
- Entrez Gene: 24882 Rat
- Entrez Gene: 60632 Zebrafish
- Omim: 165330 Human
- SwissProt: P56703 Human
- SwissProt: P17553 Mouse
see all -
Alternative names
- Int 4 antibody
- INT4 antibody
- MGC131950 antibody
see all
Images
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Anti-Wnt3 antibody (ab116222) at 1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 1 hour -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
References (1)
ab116222 has been referenced in 1 publication.
- Krivega M et al. WNT3 and membrane-associated ß-catenin regulate trophectoderm lineage differentiation in human blastocysts. Mol Hum Reprod 21:711-22 (2015). PubMed: 26108805