Anti-Wnt3 antibody (ab116222)

Reviews (1)Q&A (2)References (1)

Overview

  • Product name

    Anti-Wnt3 antibody
    See all Wnt3 primary antibodies

  • Description

    Goat polyclonal to Wnt3

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish

  • Immunogen

    Synthetic peptide:

    CGRGHNTRTEKRKEK

    , corresponding to C terminal amino acids 315-329 of Human Wnt3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human brain lysate IHC-P: Human adrenal, breast and thyroid tissues

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/2000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

  • Involvement in disease

    Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.

  • Sequence similarities

    Belongs to the Wnt family.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Int 4 antibody
    • INT4 antibody
    • MGC131950 antibody
    • MGC138321 antibody
    • MGC138323 antibody
    • Proto-oncogene Int-4 homolog antibody
    • Proto-oncogene Wnt-3 antibody
    • wingless type MMTV integration site family member 3 antibody
    • Wnt 3 proto oncogene protein antibody
    • WNT 3 proto oncogene protein precursor antibody
    • wnt3 antibody
    • WNT3_HUMAN antibody
    see all

Images

  • Western blot - Anti-Wnt3 antibody (ab116222)
    Western blot - Anti-Wnt3 antibody (ab116222)
    Anti-Wnt3 antibody (ab116222) at 1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa


    Exposure time: 1 hour
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

This product has been referenced in:

  • Krivega M  et al. WNT3 and membrane-associated ß-catenin regulate trophectoderm lineage differentiation in human blastocysts. Mol Hum Reprod 21:711-22 (2015). Read more (PubMed: 26108805) »
See 1 Publication for this product

Publishing research using ab116222? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Wnt3 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Lizard Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (4-20%, mini-PROTEAN TGX Gel)
Loading amount
60 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Dr. Oliver Griffith

Verified customer

Submitted Sep 12 2019

Question


Can you provide the blocking peptide for WNT3 antibodies ab116222?

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Answer


The blocking peptide which can be used with ab116222 is Wnt3 peptide (315-329) (ab50436).

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Question

might use ab32249, ab50341, ab116222 in mouse and ChIP and IHC-whole mount send protocol for IHC whole mount

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Answer

Thank you for contacting us. Here are the links to our Whole mount staining protocols - Protocols and troubleshooting tips for whole mount immunohistochemical staining: https://www.abcam.com/index.html?pageconfig=resource&rid=11330 and for the mouse staining in particular (also attached): https://www.abcam.com/ps/pdf/protocols/whole_mount_mouse_chick.pdf Please let me know if you like to receive the testing discounts for the 3 antibodies. Here is some more information in that regard: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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