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Anti-Wnt3 antibody (ab116222)

  • Datasheet
Reviews (1)Q&A (2)References (1)

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Western blot - Anti-Wnt3 antibody (ab116222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)

Key features and details

  • Goat polyclonal to Wnt3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Wnt3 protein (ab132336)
Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-Wnt3 antibody
    See all Wnt3 primary antibodies
  • Description

    Goat polyclonal to Wnt3
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    CGRGHNTRTEKRKEK

    , corresponding to C terminal amino acids 315-329 of Human Wnt3.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human brain lysate IHC-P: Human adrenal, breast and thyroid tissues

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Surface Molecules

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Wnt3 protein (ab132336)

Applications

Our Abpromise guarantee covers the use of ab116222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Involvement in disease

    Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA) [MIM:273395]. Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P56703 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 374142 Chicken
    • Entrez Gene: 7473 Human
    • Entrez Gene: 22415 Mouse
    • Entrez Gene: 24882 Rat
    • Entrez Gene: 60632 Zebrafish
    • Omim: 165330 Human
    • SwissProt: P56703 Human
    • SwissProt: P17553 Mouse
    • Unigene: 445884 Human
    • Unigene: 159091 Mouse
    see all
  • Alternative names

    • Int 4 antibody
    • INT4 antibody
    • MGC131950 antibody
    • MGC138321 antibody
    • MGC138323 antibody
    • Proto-oncogene Int-4 homolog antibody
    • Proto-oncogene Wnt-3 antibody
    • wingless type MMTV integration site family member 3 antibody
    • Wnt 3 proto oncogene protein antibody
    • WNT 3 proto oncogene protein precursor antibody
    • wnt3 antibody
    • WNT3_HUMAN antibody
    see all

Images

  • Western blot - Anti-Wnt3 antibody (ab116222)
    Western blot - Anti-Wnt3 antibody (ab116222)
    Anti-Wnt3 antibody (ab116222) at 1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa


    Exposure time: 1 hour
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human breast tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab116222)
    ab116222, at 3.75 µg/ml, staining Wnt3 in formalin fixed, paraffin embedded Human thyroid tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with anti-goat IgG biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab116222? Please let us know so that we can cite the reference in this datasheet.

    ab116222 has been referenced in 1 publication.

    • Krivega M  et al. WNT3 and membrane-associated ß-catenin regulate trophectoderm lineage differentiation in human blastocysts. Mol Hum Reprod 21:711-22 (2015). PubMed: 26108805

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Wnt3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Lizard Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (4-20%, mini-PROTEAN TGX Gel)
    Loading amount
    60 µg
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Oliver Griffith

    Verified customer

    Submitted Sep 12 2019

    Question


    Can you provide the blocking peptide for WNT3 antibodies ab116222?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 15 2013

    Answer


    The blocking peptide which can be used with ab116222 is Wnt3 peptide (315-329) (ab50436).

    Read More

    Abcam Scientific Support

    Answered on Mar 15 2013

    Question

    might use ab32249, ab50341, ab116222 in mouse and ChIP and IHC-whole mount send protocol for IHC whole mount

    Read More

    Abcam community

    Verified customer

    Asked on Oct 07 2011

    Answer

    Thank you for contacting us. Here are the links to our Whole mount staining protocols - Protocols and troubleshooting tips for whole mount immunohistochemical staining: https://www.abcam.com/index.html?pageconfig=resource&rid=11330 and for the mouse staining in particular (also attached): https://www.abcam.com/ps/pdf/protocols/whole_mount_mouse_chick.pdf Please let me know if you like to receive the testing discounts for the 3 antibodies. Here is some more information in that regard: For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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