Anti-Wnt3a antibody [3A6] (ab81614)
Key features and details
- Mouse monoclonal [3A6] to Wnt3a
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Wnt3a antibody [3A6]
See all Wnt3a primary antibodies -
Description
Mouse monoclonal [3A6] to Wnt3a -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).
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Positive control
- WB: A549 cell lysates, Wnt3a transfected 293T cell lysate .
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General notes
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
3A6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 89780 Human
- Omim: 606359 Human
- SwissProt: P56704 Human
- Unigene: 336930 Human
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Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab81614 has been referenced in 15 publications.
- Wu CL et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun 12:362 (2021). PubMed: 33441552
- Tian X et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
- Ackers I et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641
- Jiang S et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal 17:109 (2019). PubMed: 31462314
- Lyu X et al. microRNA-485 inhibits the malignant behaviors of retinoblastoma by directly targeting Wnt3a. Oncol Rep 41:3137-3147 (2019). PubMed: 30896857