Overview

  • Product name

    Anti-Wnt3a antibody [3A6]
    See all Wnt3a primary antibodies
  • Description

    Mouse monoclonal [3A6] to Wnt3a
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length human Wnt3a (aa19-352) purified from E. coli (NP_149122).

  • Positive control

    • WB: A549 cell lysates, Wnt3a transfected 293T cell lysate .
  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab81614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity

    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Images

  • All lanes : Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Wnt3a transfected 293T cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 39 kDa

  • All lanes : Anti-Wnt3a antibody [3A6] (ab81614) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Wnt3a transfected 293T cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 39 kDa



    This image was produced using the ascites version of this antibody. 

References

This product has been referenced in:

  • Xue C  et al. ß-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/ß-catenin pathway. Biosci Rep 38:N/A (2018). Read more (PubMed: 29358311) »
  • Wang L  et al. Expression of hepatic Wnt5a and its clinicopathological features in patients with hepatocellular carcinoma. Hepatobiliary Pancreat Dis Int 17:227-232 (2018). Read more (PubMed: 29709351) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 17 2014

Answer

Here are the answers to your questions.


1. Are there high resolution images available for the 2 IHC stainings shown on the website?
-I’ve attached higher resolution pictures as requested.

2. Have any additional sections been stained with this antibody and co-stained for cytokeratins?
&
4. What breast cancer subtype is stained in the online images?
-We regrettably do not have this information since this was outsourced to a partner hospital a couple of years ago.

3. How many different samples were stained with this antibody? Were they all positive?
-To our knowledge these two samples were the only ones tested with this antibody, and they were both positive.
I hope this is helpful. Please contact us again if you have any further questions.

Read More

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