Anti-Wnt3a antibody (ab169175)
Key features and details
- Mouse polyclonal to Wnt3a
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Wnt3a antibody
See all Wnt3a primary antibodies -
Description
Mouse polyclonal to Wnt3a -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken -
Immunogen
Recombinant full length Human Wnt3a protein (amino acids 1-385).
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Positive control
- Wnt3a transfected 293T cell lysate. Human pancreas tissue lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
- Unigene: 336930 Human
see all -
Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10µg/ml.
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Anti-Wnt3a antibody (ab169175) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 39 kDa -
All lanes : Anti-Wnt3a antibody (ab169175) at 1 µg/ml
Lane 1 : Wnt3a transfected lysate (42.35 KDa)
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 39 kDa
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab169175 has been referenced in 2 publications.
- Huang N et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). PubMed: 29433423
- Chen H et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). PubMed: 29694513