Overview

  • Product name
    Anti-Wnt3a antibody
    See all Wnt3a primary antibodies
  • Description
    Mouse polyclonal to Wnt3a
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Recombinant full length Human Wnt3a protein (amino acids 1-385).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Wnt3a transfected 293T cell lysate. Human pancreas tissue lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab169175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity
    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities
    Belongs to the Wnt family.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab169175)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab169175)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10µg/ml.
  • Western blot - Anti-Wnt3a antibody (ab169175)
    Western blot - Anti-Wnt3a antibody (ab169175)
    Anti-Wnt3a antibody (ab169175) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size: 39 kDa

  • Western blot - Anti-Wnt3a antibody (ab169175)
    Western blot - Anti-Wnt3a antibody (ab169175)
    All lanes : Anti-Wnt3a antibody (ab169175) at 1 µg/ml

    Lane 1 : Wnt3a transfected lysate (42.35 KDa)
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 39 kDa

References

This product has been referenced in:
  • Huang N  et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). Read more (PubMed: 29433423) »
  • Chen H  et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). Read more (PubMed: 29694513) »

See all 2 Publications for this product

Publishing research using ab169175? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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