Anti-Wnt3a antibody (ab169175)
Key features and details
- Mouse polyclonal to Wnt3a
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3a antibody
See all Wnt3a primary antibodies -
Description
Mouse polyclonal to Wnt3a -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken
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Immunogen
Recombinant full length Human Wnt3a protein (amino acids 1-385).
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Positive control
- Wnt3a transfected 293T cell lysate. Human pancreas tissue lysate. HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab169175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
- Unigene: 336930 Human
see all -
Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab169175)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10µg/ml.
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Western blot - Anti-Wnt3a antibody (ab169175)Anti-Wnt3a antibody (ab169175) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 39 kDa -
Western blot - Anti-Wnt3a antibody (ab169175)All lanes : Anti-Wnt3a antibody (ab169175) at 1 µg/ml
Lane 1 : Wnt3a transfected lysate (42.35 KDa)
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 39 kDa
Protocols
Datasheets and documents
References (2)
ab169175 has been referenced in 2 publications.
- Huang N et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). PubMed: 29433423
- Chen H et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). PubMed: 29694513
