Anti-Wnt3a antibody (ab169175)

Product name

Anti-Wnt3a antibody
See all Wnt3a primary antibodies
Description

Mouse polyclonal to Wnt3a
Host species

Mouse
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Chicken
Immunogen

Recombinant full length Human Wnt3a protein (amino acids 1-385).

Run BLAST with Run BLAST with
Positive control
- Wnt3a transfected 293T cell lysate. Human pancreas tissue lysate. HeLa cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.20
Constituent: 99% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab169175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF		Use a concentration of 10 µg/ml.

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Tissue specificity

Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Sequence similarities

Belongs to the Wnt family.
Cellular localization

Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accession P56704 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
- Unigene: 336930 Human
- Unigene: 1367 Mouse
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Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
- Wnt 3a antibody
- wnt3a antibody
- WNT3A protein antibody
- WNT3A_HUMAN antibody
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Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab169175)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10µg/ml.
Western blot - Anti-Wnt3a antibody (ab169175)

Anti-Wnt3a antibody (ab169175) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

Predicted band size: 39 kDa
Western blot - Anti-Wnt3a antibody (ab169175)

All lanes : Anti-Wnt3a antibody (ab169175) at 1 µg/ml

Lane 1 : Wnt3a transfected lysate (42.35 KDa)
Lane 2 : Non-transfected lysate

Lysates/proteins at 15 µl per lane.

Predicted band size: 39 kDa

Huang N et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). Read more (PubMed: 29433423) »
Chen H et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). Read more (PubMed: 29694513) »

Publishing research using ab169175? Please let us know so that we can cite the reference in this datasheet.

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