Key features and details
- Mouse polyclonal to Wnt3a
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Wnt3a antibody
See all Wnt3a primary antibodies
DescriptionMouse polyclonal to Wnt3a
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken
- Wnt3a transfected 293T cell lysate. Human pancreas tissue lysate. HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Tissue specificityModerately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10µg/ml.
Anti-Wnt3a antibody (ab169175) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 39 kDa
All lanes : Anti-Wnt3a antibody (ab169175) at 1 µg/ml
Lane 1 : Wnt3a transfected lysate (42.35 KDa)
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 39 kDa
ab169175 has been referenced in 2 publications.
- Huang N et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). PubMed: 29433423
- Chen H et al. miR-27a protects human mitral valve interstitial cell from TNF-a-induced inflammatory injury via up-regulation of NELL-1. Braz J Med Biol Res 51:e6997 (2018). PubMed: 29694513