Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt3a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity


    Predicted to work with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Wnt3a. (E.coli derived; highly pure (>98%)).
    Database link: P56704

  • Positive control

    • WB: Recombinant human Wnt3a

Properties

  • Form

    Lyophilised:For lot specific reconstitution information please contact our Scientific Support Team.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab231365 was purified by affinity chromatography employing immobilized human Wnt3a matrix.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Wnt3a (Biotin) (ab54479).
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa.

Used in conjunction with compatible secondary reagents the detection limit for recombinant human Wnt-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity

    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein Wnt 3a Precursor antibody
    • Protein Wnt-3a antibody
    • Wingless type MMTV integration site family member 3A antibody
    • Wnt 3a antibody
    • wnt3a antibody
    • WNT3A protein antibody
    • WNT3A_HUMAN antibody
    see all

Images

  • All lanes : Anti-Wnt3a antibody (ab231365)

    All lanes : Recombinant human Wnt3a

    Performed under non-reducing conditions.

    Predicted band size: 39 kDa



    Used in conjunction with compatible secondary reagents the detection limit for recombinant human Wnt-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

  • All lanes : Anti-Wnt3a antibody (ab231365)

    All lanes : Recombinant human Wnt3a

    Performed under reducing conditions.

    Predicted band size: 39 kDa



    Used in conjunction with compatible secondary reagents the detection limit for recombinant human Wnt-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

  • ab231365, in conjunction with ab54479 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.

References

ab231365 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab231365.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up