Anti-Wnt3a antibody (ab28472)
Key features and details
- Rabbit polyclonal to Wnt3a
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Wnt3a antibody
See all Wnt3a primary antibodies -
Description
Rabbit polyclonal to Wnt3a -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab28472 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab219412.
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Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis, Zebrafish, a wide range of other species -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab23327) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt3a peptide (ab23327).
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt3a peptide (ab23327). |
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
Tissue specificity
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Entrez Gene: 378679 Xenopus laevis
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
see all -
Alternative names
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
Images
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Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.
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ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (114)
ab28472 has been referenced in 114 publications.
- Ford EA et al. Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation†. Biol Reprod 106:503-514 (2022). PubMed: 34673933
- Fletcher AJ et al. Microcalcification and Thoracic Aortopathy: A Window Into Disease Severity. Arterioscler Thromb Vasc Biol 42:1048-1059 (2022). PubMed: 35770666
- Li C et al. Chalcone 9X Contributed to Repressing Glioma Cell Growth and Migration and Inducing Cell Apoptosis by Reducing FOXM1 Expression In Vitro and Repressing Tumor Growth In Vivo. Biomed Res Int 2022:8638085 (2022). PubMed: 35978634
- Li Y et al. Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/β-Catenin Signaling. Cell Stem Cell 28:63-78.e7 (2021). PubMed: 33053374
- Shu Y et al. Paired-like homeodomain transcription factor 2 strengthens chemoresistance in colorectal cancer by activating the Wnt/β-catenin axis. Neoplasma 68:557-566 (2021). PubMed: 33780262