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1) ab28472 rabbit pAb to wnt3a
2) there is a clear band around 55kDa, not as described 68kDa
3)One of my samples are from mouse tissue, the other is from myocytes
,Lysis buffer is Ripa buffer, and inhibitors are lea, Aprop, ABESF
4) I use both 8% and 10% gel
5) primary antibody i use 1:1000 dilution in 5%BSA, incubate 4 degree overnight
6) second antibody is anti-rabbit 1:3000, incubate room temp. 1hour
7) i use GE ECL plus western blotting detection.
Asked on Mar 27 2012
Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Wnt3a antibody.
The details you have kindly provided will enable us to investigate this case for you and also gives us vital information for our monitoring of product quality.
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Although post-translational modifications such as palmitoylation or glycosylation (as described for Wnt3a) can alter the electrophoretic mobility of the protein so that it runs at a higher molecular weight, I am not sure whether this is the cause of the problem you observed. Also, the protocol seems absolutely fine to me andI am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.
I apologise for the inconvenience and am pleased to offer you a free of charge replacement. The replacement could be a new vial of ab28472 or our alternative Wnt3a antibody ab19925 or any other primary antibody of your choice. Otherwise, I would be pleased to arrange acredit note in compensation.
In order to arrange this, could you please let me know one of the following: the order number, the PO number, the date of purchase or at least the address of your lab?
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.
Answered on Mar 27 2012