Other product details
Host species: Donkey
Conjugation: Alexa Fluor® 594
Maleic acid blocking solution is: 150mM maleic acid, 100mM NaCl, 2% blocking reagent (roche), pH7.5, 0.1% Tween-20.
Image shows data for Wnt3a by wholemount IHC of a representative 24hpf zebrafish embryo. When compared to the expression pattern of wnt3a at 24hpf detected by in situ hybridization (as published in Nyholm et al., 2007) this antibody gives high background, but some staining is observed in expected tissue.
Nyholm MK, Wu SF, Dorsky RI and Y Grinblat (2007) The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum. Development. 134: 735-746.
Ms. Emerald Butko
Submitted Mar 09 2012