Key features and details
- Rabbit polyclonal to Wnt3a (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Wnt3a antibody (Biotin)
See all Wnt3a primary antibodies
DescriptionRabbit polyclonal to Wnt3a (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein (Human),>98% pure
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Our Abpromise guarantee covers the use of ab54479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Use 100 µl antibody solution per well. This amount allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Wnt3a.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 39 kDa.
The detection limit is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Tissue specificityModerately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
ab54479 has been referenced in 2 publications.