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RecombinantRabMAb

Recombinant Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

Reviews (1) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
  • Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
  • Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
  • Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21889] to Wnt3a - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC HRP PE Unconjugated

Overview

  • Product name

    Anti-Wnt3a antibody [EPR21889] - BSA and Azide free
    See all Wnt3a primary antibodies

  • Description

    Rabbit monoclonal [EPR21889] to Wnt3a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat E14.5 tissue.

  • General notes

    ab234099 is the carrier-free version of ab219412.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab234099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

  • Tissue specificity

    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.

  • Sequence similarities

    Belongs to the Wnt family.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • Protein Wnt 3a Precursor antibody
      • Protein Wnt-3a antibody
      • Wingless type MMTV integration site family member 3A antibody
      • Wnt 3a antibody
      • wnt3a antibody
      • WNT3A protein antibody
      • WNT3A_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

      Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of mouse E14.5 (PMID: 15509764; PMID: 16410414) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219412).

    • Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cell line (right) labeling  Wnt3a with ab219412 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. 

      Two Wnt3a-expressing populations can be seen labeled in this stably transfected cell line. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219412). 

       

       

    • Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

      Wnt3a was immunoprecipitated from 0.35 mg L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate with ab219412 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
      Lane 1: L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate 10 µg (Input).
      Lane 2: ab219412 IP in L Wnt-3A whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219412 in L Wnt-3a whole cell lysate.
      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219412).

    • Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cells labeling Wnt3a with ab219412 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in L Wnt-3A cell line. The nuclear counter stain is DAPI (blue).
      Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
      The negative control is the secondary antibody only.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219412).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

      Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of rat E14.5 (PMID: 15509764; PMID: 16410414) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219412).

       

    • Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)
      Anti-Wnt3a antibody [EPR21889] - BSA and Azide free (ab234099)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab234099? Please let us know so that we can cite the reference in this datasheet.

    ab234099 has been referenced in 3 publications.

    • Lin Z & Liu J lncRNA DQ786243 promotes hepatocellular carcinoma cell invasion and proliferation by regulating the miR-15p-5p/Wnt3A axis. Mol Med Rep 23:N/A (2021). PubMed: 33760109
    • Yao J  et al. Long non-coding RNA FEZF1-AS1 promotes the proliferation and metastasis of hepatocellular carcinoma via targeting miR-107/Wnt/ß-catenin axis. Aging (Albany NY) 13:13726-13738 (2021). PubMed: 34023817
    • Li W  et al. Effects of Environmental pH on the Growth of Gastric Cancer Cells. Gastroenterol Res Pract 2020:3245359 (2020). PubMed: 32211041

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-Wnt3a antibody [EPR21889] - BSA and Azide free

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (oral cavity)
    Permeabilization
    Yes - Triton
    Specification
    oral cavity
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Tina Yuan

    Verified customer

    Submitted Sep 11 2018

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