Recombinant Anti-Wnt4 antibody [9HCLC] (ab277798)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [9HCLC] to Wnt4
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Wnt4 antibody [9HCLC]
See all Wnt4 primary antibodies -
Description
Rabbit recombinant multiclonal [9HCLC] to Wnt4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: Jurkat, K562, HCT 116, PANC1 and HeLa whole cell extracts; A-431 cells. ICC/IF: HepG2 cells.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
9HCLC -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.5 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 39 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 39 kDa. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue. -
Involvement in disease
Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330]. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 54361 Human
- Omim: 603490 Human
- SwissProt: P56705 Human
- Unigene: 25766 Human
- Unigene: 611722 Human
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Alternative names
- MGC123964 antibody
- Protein Wnt-4 antibody
- RP23-246F18.1 antibody
see all
Images
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All lanes : Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/ml
Lane 1 : Jurkat whole cell extracts
Lane 2 : K562 whole cell extracts
Lane 3 : HCT 116 whole cell extracts
Lane 4 : PANC1 whole cell extracts
Lane 5 : HeLa whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution
Predicted band size: 39 kDa -
All lanes : Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/ml
Lane 1 : A-431 cells untransfected
Lane 2 : A-431 cells transfected with non-specific scrambled siRNA
Lane 3 : A-431 whole cell extract with WNT4 knockdown
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/4000 dilution
Predicted band size: 39 kDa -
Immunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of Wnt4 using Anti-Wnt4 Recombinant Rabbit Multiclonal Antibody (ab277798, 0.5 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Wnt4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Wnt4 protein. Panel e) represents control cells with no primary Antibody to assess background.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab277798 has been referenced in 1 publication.
- Ni C et al. Wnt4 prevents apoptosis and inflammation of dental pulp cells induced by LPS by inhibiting the IKK/NF‑κB pathway. Exp Ther Med 25:75 (2023). PubMed: 36684653