Anti-Wnt4 antibody (ab169592)
Key features and details
- Mouse polyclonal to Wnt4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt4 antibody
See all Wnt4 primary antibodies -
Description
Mouse polyclonal to Wnt4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
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Immunogen
Full length protein, corresponding to amino acids 1-351 of Human Wnt4 (Accession number: NP_110388.2; UniProt ID: P56705).
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Positive control
- Wnt4 transfected 293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue. -
Involvement in disease
Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330]. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 54361 Human
- Entrez Gene: 22417 Mouse
- Entrez Gene: 84426 Rat
- Entrez Gene: 397706 Xenopus laevis
- Omim: 603490 Human
- SwissProt: P49337 Chicken
- SwissProt: P56705 Human
- SwissProt: P22724 Mouse
see all -
Alternative names
- MGC123964 antibody
- Protein Wnt-4 antibody
- RP23-246F18.1 antibody
see all
Images
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Western blot - Anti-Wnt4 antibody (ab169592)All lanes : Anti-Wnt4 antibody (ab169592) at 1 µg/ml
Lane 1 : Wnt4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 39 kDa
Datasheets and documents
References (1)
ab169592 has been referenced in 1 publication.
- Wang W et al. Effects of melatonin on diabetic nephropathy rats via Wnt/ß-catenin signaling pathway and TGF-ß-Smad signaling pathway. Int J Clin Exp Pathol 11:2488-2496 (2018). PubMed: 31938361
