Product nameAnti-Wnt4 antibody
See all Wnt4 primary antibodies
DescriptionRabbit polyclonal to Wnt4
Tested applicationsSuitable for: IHC-P, Flow Cyt, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Horse, Human
Synthetic peptide corresponding to Human Wnt4 aa 211-239 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 54361
- WB: Mouse bladder tissue lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt: MB468 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesab91226 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab91226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Predicted molecular weight: 39 kDa.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
Involvement in diseaseDefects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome) [MIM:277000]; also called Mayer-Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls.
Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL) [MIM:611812]; also known as SERKAL syndrome.
Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL) [MIM:158330].
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- MGC123964 antibody
- Protein Wnt-4 antibody
- RP23-246F18.1 antibody
Anti-Wnt4 antibody (ab91226) at 1/1000 dilution + Mouse bladder tissue lysate at 35 µg
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 39 kDa
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraformaldehyde-fixed frozen murine thymus tissue sections, labelling Wnt4 with ab91226 at a dilution of 1/20 incubated for 72 hours at 25°C in a 1% BSA & 3% donkey serum solution in PBST. Permeabilsation was with PBST including 0.1% Triton X-100. Blocking was with the BSA & donkey serum ab91226 was diluted in, incubated for 1 hour at 25°C. The secondary used was a donkey anti-rabbit polyclonal Alexa Fluor® 488 used at 1/1000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling Wnt4 with ab91226. A peroxidase-conjugated secondary antibody was used, followed by DAB staining.
ICC/IF image of ab91226 stained MCF7 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91226, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow cytometry analysis of MDA-MB468 cells labelling Wnt4 (green) with ab91226 compared to a negative control (blue). A FITC-conjugated goat anit-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- Liu S et al. Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells. Stem Cells Int 2017:7168687 (2017). WB . Read more (PubMed: 28194187) »
- Sun X et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). Read more (PubMed: 28147310) »