Anti-Wnt5a antibody (ab227229)

Rabbit polyclonal Wnt5a antibody. Validated in WB and tested in Rat, Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt5a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis
  • Immunogen

    Recombinant fragment within Human Wnt5a (internal sequence). The exact sequence is proprietary.
    Database link: P41221

  • Positive control

    • WB: Rat heart extract; Wnt5a transfected HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • Tissue specificity

    Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).
  • Sequence similarities

    Belongs to the Wnt family.
  • Post-translational
    modifications

    Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.
    Glycosylation is necessary for secretion but not for activity.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • hWNT 5A antibody
    • hWNT5A antibody
    • Protein Wnt 5a antibody
    • Protein Wnt-5a antibody
    • Protein Wnt5a antibody
    • Wingless type MMTV integration site family member 5A antibody
    • Wnt 5a antibody
    • WNT 5A protein antibody
    • WNT 5A protein precursor antibody
    • WNT5A antibody
    • WNT5A protein precursor antibody
    • WNT5A_HUMAN antibody
    see all

Images

  • All lanes : Anti-Wnt5a antibody (ab227229) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Wnt5a transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

  • Anti-Wnt5a antibody (ab227229) at 1/500 dilution + Rat heart extract at 50 µg

    Predicted band size: 42 kDa



    10% SDS-PAGE gel.

References

ab227229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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