Anti-Wnt5b antibody (ab169071)
Key features and details
- Mouse polyclonal to Wnt5b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt5b antibody
See all Wnt5b primary antibodies -
Description
Mouse polyclonal to Wnt5b -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length protein, corresponding to amino acids 1-359 of Human Wnt5b (NP_110402.2).
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Positive control
- Wnt5b-transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 81029 Human
- Entrez Gene: 22419 Mouse
- Entrez Gene: 100174132 Orangutan
- Omim: 606361 Human
- SwissProt: Q9H1J7 Human
- SwissProt: P22726 Mouse
- SwissProt: Q5NVK2 Orangutan
- Unigene: 306051 Human
see all -
Alternative names
- MGC2648 antibody
- Protein Wnt-5b antibody
- Wingless type MMTV integration site family, member 5B antibody
see all
Images
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Western blot - Anti-Wnt5b antibody (ab169071)All lanes : Anti-Wnt5b antibody (ab169071) at 1 µg/ml
Lane 1 : Wnt5b-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Datasheets and documents
References (0)
ab169071 has not yet been referenced specifically in any publications.
