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    wnt5b-antibody-ab169071.pdf

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Stem Cells Signaling Pathways Wnt Secreted
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Anti-Wnt5b antibody (ab169071)

  • Datasheet
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Western blot - Anti-Wnt5b antibody (ab169071)

    Key features and details

    • Mouse polyclonal to Wnt5b
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Wnt5b protein (ab164311)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Wnt5b antibody
      See all Wnt5b primary antibodies
    • Description

      Mouse polyclonal to Wnt5b
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Full length protein, corresponding to amino acids 1-359 of Human Wnt5b (NP_110402.2).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Wnt5b-transfected 293T cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Secreted

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human Wnt5b protein (ab164311)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab169071 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Orangutan
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.

    Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Sequence similarities

      Belongs to the Wnt family.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession Q9H1J7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 81029 Human
      • Entrez Gene: 22419 Mouse
      • Entrez Gene: 100174132 Orangutan
      • Omim: 606361 Human
      • SwissProt: Q9H1J7 Human
      • SwissProt: P22726 Mouse
      • SwissProt: Q5NVK2 Orangutan
      • Unigene: 306051 Human
      • Unigene: 321818 Mouse
      see all
    • Alternative names

      • MGC2648 antibody
      • Protein Wnt-5b antibody
      • Wingless type MMTV integration site family, member 5B antibody
      • WNT 5B antibody
      • WNT 5B protein antibody
      • Wnt family member 5B antibody
      • Wnt5b antibody
      • WNT5B protein antibody
      • WNT5B_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Wnt5b antibody (ab169071)
      Western blot - Anti-Wnt5b antibody (ab169071)
      All lanes : Anti-Wnt5b antibody (ab169071) at 1 µg/ml

      Lane 1 : Wnt5b-transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 40 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab169071? Please let us know so that we can cite the reference in this datasheet.

    ab169071 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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