Recombinant
RabMAb

Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

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Overview

  • Product name
    Anti-Wnt5b antibody [EPR7135(B)]
    See all Wnt5b primary antibodies
  • Description
    Rabbit monoclonal [EPR7135(B)] to Wnt5b
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Wnt5b aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HT-1376 cell lysate, HepG2 cell lysate, 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7135(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function
      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Sequence similarities
      Belongs to the Wnt family.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
      • Alternative names
        • MGC2648 antibody
        • Protein Wnt-5b antibody
        • Wingless type MMTV integration site family, member 5B antibody
        • WNT 5B antibody
        • WNT 5B protein antibody
        • Wnt family member 5B antibody
        • Wnt5b antibody
        • WNT5B protein antibody
        • WNT5B_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution

        Lane 1 : HT-1376 cell lysate

        Lane 2 : HepG2 cell lysate

        Lane 3 : 293T cell lysate

        Lane 4 : SH-SY5Y cell lysate


        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 40 kDa

      References

      ab124818 has not yet been referenced specifically in any publications.

      Publishing research using ab124818? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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