Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7135(B)] to Wnt5b
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-Wnt5b antibody [EPR7135(B)]
See all Wnt5b primary antibodies
DescriptionRabbit monoclonal [EPR7135(B)] to Wnt5b
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Wnt5b aa 300-400 (C terminal). The exact sequence is proprietary.
- HT-1376 cell lysate, HepG2 whole cell lysate (ab7900), 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- MGC2648 antibody
- Protein Wnt-5b antibody
- Wingless type MMTV integration site family, member 5B antibody
All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa
ab124818 has been referenced in 2 publications.
- Jiang S et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal 17:109 (2019). PubMed: 31462314
- Hino M et al. Transforming growth factor-ß1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. J Biochem 159:631-40 (2016). PubMed: 26861993