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Stem Cells Signaling Pathways Wnt Secreted
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RecombinantRabMAb

Recombinant Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

  • Datasheet
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Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
  • Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7135(B)] to Wnt5b
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human Wnt5b protein (ab164311)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Wnt5b antibody [EPR7135(B)]
    See all Wnt5b primary antibodies
  • Description

    Rabbit monoclonal [EPR7135(B)] to Wnt5b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Wnt5b aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HT-1376 cell lysate, HepG2 whole cell lysate (ab7900), 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7135(B)
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Associated products

  • Alternative Versions

    • Anti-Wnt5b antibody [EPR7135(B)] - BSA and Azide free (ab181723)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Wnt5b protein (ab164311)

Applications

Our Abpromise guarantee covers the use of ab124818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
    • Sequence similarities

      Belongs to the Wnt family.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession Q9H1J7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 81029 Human
      • Omim: 606361 Human
      • SwissProt: Q9H1J7 Human
      • Unigene: 306051 Human
      • Alternative names

        • MGC2648 antibody
        • Protein Wnt-5b antibody
        • Wingless type MMTV integration site family, member 5B antibody
        • WNT 5B antibody
        • WNT 5B protein antibody
        • Wnt family member 5B antibody
        • Wnt5b antibody
        • WNT5B protein antibody
        • WNT5B_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution

        Lane 1 : HT-1376 cell lysate

        Lane 2 : HepG2 cell lysate

        Lane 3 : 293T cell lysate

        Lane 4 : SH-SY5Y cell lysate


        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 40 kDa

      • Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab124818? Please let us know so that we can cite the reference in this datasheet.

      ab124818 has been referenced in 2 publications.

      • Jiang S  et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal 17:109 (2019). PubMed: 31462314
      • Hino M  et al. Transforming growth factor-ß1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. J Biochem 159:631-40 (2016). PubMed: 26861993

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab124818.
      Please use the links above to contact us or submit feedback about this product.

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      For licensing inquiries, please contact partnerships@abcam.com

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