Recombinant Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Wnt5b antibody [EPR7135(B)]
See all Wnt5b primary antibodies -
Description
Rabbit monoclonal [EPR7135(B)] to Wnt5b -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Wnt5b aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- HT-1376 cell lysate, HepG2 whole cell lysate (ab7900), 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7135(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 81029 Human
- Omim: 606361 Human
- SwissProt: Q9H1J7 Human
- Unigene: 306051 Human
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Alternative names
- MGC2648 antibody
- Protein Wnt-5b antibody
- Wingless type MMTV integration site family, member 5B antibody
see all
Images
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![Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)](https://www.abcam.com/ps/products/124/ab124818/Images/ab124818-138738-anti-wnt5b-antibody-epr7135b-western-blot.jpg)
Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa
Datasheets and documents
References
This product has been referenced in:
- Hino M et al. Transforming growth factor-ß1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. J Biochem 159:631-40 (2016). Read more (PubMed: 26861993) »
