Recombinant
RabMAb

Recombinant Anti-Wnt5b antibody [EPR7135(B)] (ab124818)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7135(B)] to Wnt5b
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Wnt5b antibody [EPR7135(B)]
    See all Wnt5b primary antibodies

  • Description

    Rabbit monoclonal [EPR7135(B)] to Wnt5b

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Wnt5b aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HT-1376 cell lysate, HepG2 whole cell lysate (ab7900), 293T cell lysates, and SH-SY5Y cell lysate. HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7135(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

    • Sequence similarities

      Belongs to the Wnt family.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • MGC2648 antibody
        • Protein Wnt-5b antibody
        • Wingless type MMTV integration site family, member 5B antibody
        • WNT 5B antibody
        • WNT 5B protein antibody
        • Wnt family member 5B antibody
        • Wnt5b antibody
        • WNT5B protein antibody
        • WNT5B_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        Western blot - Anti-Wnt5b antibody [EPR7135(B)] (ab124818)
        All lanes : Anti-Wnt5b antibody [EPR7135(B)] (ab124818) at 1/1000 dilution

        Lane 1 : HT-1376 cell lysate

        Lane 2 : HepG2 cell lysate

        Lane 3 : 293T cell lysate

        Lane 4 : SH-SY5Y cell lysate


        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 40 kDa

      References

      Publishing research using ab124818? Please let us know so that we can cite the reference in this datasheet.

      ab124818 has been referenced in 2 publications.

      • Jiang S  et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal 17:109 (2019). PubMed: 31462314
      • Hino M  et al. Transforming growth factor-ß1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. J Biochem 159:631-40 (2016). PubMed: 26861993

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