Recombinant Anti-Wnt6 antibody [EPR9244] (ab154144)
![Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)](https://www.abcam.com/ps/products/154/ab154144/Images/ab154144-160064-anti-wnt6-antibody-epr9244-western-blot.jpg "Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9244] to Wnt6
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt6 antibody [EPR9244]
See all Wnt6 primary antibodies -
Description
Rabbit monoclonal [EPR9244] to Wnt6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Wnt6.
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Positive control
- HeLa, Jurkat, SH-SY5Y, C6, RAW 264.7, PC12, NIH 3T3 and Neuro 2a cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
Sequence similarities
Belongs to the Wnt family. -
Post-translational
modificationsPalmitoylation at Ser-228 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-76. Palmitoylation is necessary for proper trafficking to cell surface. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7475 Human
- Entrez Gene: 22420 Mouse
- Entrez Gene: 316526 Rat
- Omim: 604663 Human
- SwissProt: Q9Y6F9 Human
- SwissProt: P22727 Mouse
- Unigene: 29764 Human
- Unigene: 268282 Mouse
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Alternative names
- Protein Wnt 6 antibody
- Protein Wnt-6 antibody
- Protein Wnt-6 precursor antibody
see all
Images
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Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)All lanes : Anti-Wnt6 antibody [EPR9244] (ab154144) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa -
![Anti-Wnt6 antibody [EPR9244] (ab154144)](https://www.abcam.com/ps/products/154/ab154144/Images/ab154144-2-benefits-of-recombinant-antibodies.png)
Anti-Wnt6 antibody [EPR9244] (ab154144)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154144 has been referenced in 2 publications.
- Gonçalves CS et al. WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma. Theranostics 8:4805-4823 (2018). PubMed: 30279739
- Jiang Z et al. Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the ß-catenin pathway. Exp Ther Med 14:5339-5344 (2017). PubMed: 29285061