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    wnt6-antibody-epr9244-ab154144.pdf

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RecombinantRabMAb

Recombinant Anti-Wnt6 antibody [EPR9244] (ab154144)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)
  • Anti-Wnt6 antibody [EPR9244] (ab154144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9244] to Wnt6
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Wnt6 protein (ab159813)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Wnt6 antibody [EPR9244]
    See all Wnt6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9244] to Wnt6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Wnt6.

  • Positive control

    • HeLa, Jurkat, SH-SY5Y, C6, RAW 264.7, PC12, NIH 3T3 and Neuro 2a cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9244
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTC & Wnt pathway
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-Wnt6 antibody [EPR9244] - BSA and Azide free (ab249068)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Wnt6 protein (ab159813)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Sequence similarities

    Belongs to the Wnt family.
  • Post-translational
    modifications

    Palmitoylation at Ser-228 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-76. Palmitoylation is necessary for proper trafficking to cell surface.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession Q9Y6F9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7475 Human
    • Entrez Gene: 22420 Mouse
    • Entrez Gene: 316526 Rat
    • Omim: 604663 Human
    • SwissProt: Q9Y6F9 Human
    • SwissProt: P22727 Mouse
    • Unigene: 29764 Human
    • Unigene: 268282 Mouse
    • Alternative names

      • Protein Wnt 6 antibody
      • Protein Wnt-6 antibody
      • Protein Wnt-6 precursor antibody
      • Wingless type MMTV integration site family, member 6 antibody
      • wnt 6 antibody
      • Wnt6 antibody
      • WNT6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)
      Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144)
      All lanes : Anti-Wnt6 antibody [EPR9244] (ab154144) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH 3T3 cell lysate
      Lane 8 : Neuro 2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 40 kDa

    • Anti-Wnt6 antibody [EPR9244] (ab154144)
      Anti-Wnt6 antibody [EPR9244] (ab154144)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab154144? Please let us know so that we can cite the reference in this datasheet.

    ab154144 has been referenced in 2 publications.

    • Gonçalves CS  et al. WNT6 is a novel oncogenic prognostic biomarker in human glioblastoma. Theranostics 8:4805-4823 (2018). PubMed: 30279739
    • Jiang Z  et al. Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the ß-catenin pathway. Exp Ther Med 14:5339-5344 (2017). PubMed: 29285061

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    zunächst einmal besten Dank für die Informationen! Sofern ich über die Promotion-Aktion bestelle mit einer wieviel-prozentigen Preisreduktion kann ich rechnen?

    Beste Grüsse

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Mit der RabMab Aktion bekommen Sie bis zum 16. Dezember beim Kauf eines primären Antikörpers einen RabMab Antikörper kostenlos. Dafür müssen Sie nur den Folgenden Code bei der Bestellung angeben: RABMAB-XBSMF.

    Wir haben einen Link auf der Abcam Homepage unter dem Sie weiterer Informationen über diese Angebot und die RabMab monoklonalen Antikörper nachlesen können.

    https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Ich hoffe dies hilft Ihnen weiter. Bitte zögern Sie nicht mich erneut zu kontaktieren sollten Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    Sehr geehrte Damen und Herren,
    werden Antikörper von Epitomics (Anti-WNT6, clone EPR9244 ; BestellNr.
    6669-1) von Ihnen vertrieben? Falls ja, mit welchem Preis sind für 100µl
    des genannten AK zu rechnen?
    Vielen Dank im vorraus.
    Beste Grüsse

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2012

    Answer

    Vielen Dank für Ihre Anfrage.

    Ich habe mich nach dem WNT6 Antikörper von Epitomics für Sie erkundigt. Wir bieten diesen jetzt an unter folgender Nummer in unserem Katalog:


    https://www.abcam.com/wnt6-antibody-epr9244-ab154144.html

    Ich hoffe dies hilft Ihnen weiter. Wenn Sie diesen Antikörper bis zum 16/12/12 bei und bestellen könnte unsere RabMab Aktion von Interesse für Sie sein. Für mehr Informationen schauen Sie bittehttps://www.abcam.com/index.html?pageconfig=resource&rid=15447.

    Sollten Sie weitere Fragen haben zögern Sie bitte nicht sich wieder bei mir zu melden.

    Read More

    Abcam Scientific Support

    Answered on Dec 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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