Key features and details
- Rabbit polyclonal to Wnt7a
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Wnt7a antibody
See all Wnt7a primary antibodies
DescriptionRabbit polyclonal to Wnt7a
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat brain and human colon carcinoma lysates. Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.
Tissue specificityExpression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
Involvement in diseaseDefects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt-7a antibody
- Protein Wnt-7a precursor antibody
- Proto oncogene Wnt7a protein antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue, labeling Wnt7a using ab217844 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-Wnt7a antibody (ab217844) at 1/200 dilution
Lane 1 : rat brain lysates
Lane 2 : human colon carcinoma lysates
All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
ab217844 has been referenced in 1 publication.
- Wu DJ et al. High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma. Sci Rep 8:15792 (2018). PubMed: 30361522