Recombinant Anti-Wnt7a antibody [EPR23471-125] (ab274321)
![Western blot - Anti-Wnt7a antibody [EPR23471-125] (ab274321)](https://www.abcam.com/ps/products/274/ab274321/Images/ab274321-396981-anti-wnt7a-antibody-epr23471125-western-blot-hacat1.jpg "Western blot - Anti-Wnt7a antibody [EPR23471-125] (ab274321)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23471-125] to Wnt7a
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Wnt7a antibody [EPR23471-125]
See all Wnt7a primary antibodies -
Description
Rabbit monoclonal [EPR23471-125] to Wnt7a -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HaCaT and BxPC-3 whole cell lysates; Mouse P7 hippocampus tissue lysate. ICC/IF: HaCaT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23471-125 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab274321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
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| WB |
1/1000. Detects a band of approximately 40, 42 kDa (predicted molecular weight: 39 kDa).
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| Notes |
|---|
|
ICC/IF
1/50. |
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WB
1/1000. Detects a band of approximately 40, 42 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. -
Tissue specificity
Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. -
Involvement in disease
Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7476 Human
- Entrez Gene: 22421 Mouse
- Omim: 601570 Human
- SwissProt: O00755 Human
- SwissProt: P24383 Mouse
- Unigene: 72290 Human
- Unigene: 56964 Mouse
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Alternative names
- Protein Wnt-7a antibody
- Protein Wnt-7a precursor antibody
- Proto oncogene Wnt7a protein antibody
see all
Images
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Western blot - Anti-Wnt7a antibody [EPR23471-125] (ab274321)All lanes : Anti-Wnt7a antibody [EPR23471-125] (ab274321) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Mouse P7 hippocampus tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 42/40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 25978974, 30143647, 30770219).
Negative control: HeLa cells (PMID: 25978974).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] (ab274321)](https://www.abcam.com/ps/products/274/ab274321/Images/ab274321-1-anti-wnt7a-antibody-epr23471125-immunocytochemistry-hacat1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody [EPR23471-125] (ab274321)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HaCaT (human skin keratinocyte) and HeLa (human cervix adenocarcinoma epithelial cell) whole cells labelling Wnt7a with ab274321 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in HaCaT cells. Low expression cell line: HeLa cells (PMID: 25978974). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-Wnt7a antibody [EPR23471-125] (ab274321)](https://www.abcam.com/ps/products/274/ab274321/Images/ab274321-3-benefits-of-recombinant-antibodies.png)
Anti-Wnt7a antibody [EPR23471-125] (ab274321)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab274321 has not yet been referenced specifically in any publications.