Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt7b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 87-307 of Human Wnt7b (Uniprot ID: P56706).

  • Positive control

    • NT2D1 whole cell lysate; Wnt7b transfected 293T cell lysate; Human Ovarian carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificity

    Moderately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Form

    Wnt7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid modified and are ligands for members of the frizzled family of receptors, which mediate cell-cell communication during development. Wnt7b is thought to modulate several important signaling pathways in the lung which results in the coordinated proliferation of adjacent epithelial and mesenchymal cells to stimulate organ growth.
  • Alternative names

    • Protein Wnt-7b antibody
    • Wingless related MMTV integration site 7B antibody
    • Wingless type MMTV integration site family member 7B antibody
    • WNT antibody
    • WNT7B antibody
    • WNT7B_HUMAN antibody
    see all

Images

  • Anti-Wnt7b antibody (ab155313) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 39 kDa



    10% SDS PAGE
  • All lanes : Anti-Wnt7b antibody (ab155313) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate, non-transfected
    Lane 2 : 293T whole cell lysate, transfected with WNT7b

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Ovarian carcinoma tissue, labeling Wnt7b with ab155313 at 1/250 dilution.

References

This product has been referenced in:

  • Vallon M  et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). Read more (PubMed: 30304675) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Robert Kupp

Verified customer

Submitted Jun 27 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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