Key features and details
- Rabbit polyclonal to Wnt7b
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Wnt7b antibody
See all Wnt7b primary antibodies
DescriptionRabbit polyclonal to Wnt7b
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 87-307 of Human Wnt7b (Uniprot ID: P56706).
- WB: NT2D1; Wnt7b transfected HEK-293T whole cell lysate. IHC-P: human ovarian carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/10000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Tissue specificityModerately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
FormWnt7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid modified and are ligands for members of the frizzled family of receptors, which mediate cell-cell communication during development. Wnt7b is thought to modulate several important signaling pathways in the lung which results in the coordinated proliferation of adjacent epithelial and mesenchymal cells to stimulate organ growth.
- Protein Wnt-7b antibody
- Wingless related MMTV integration site 7B antibody
- Wingless type MMTV integration site family member 7B antibody
Anti-Wnt7b antibody (ab155313) at 1/1000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue stained for Wnt7b using ab155313 at 1/250 dilution.
Antigen Retrieval:Trilogy™(EDTA based, pH 8.0) buffer, 15min.
All lanes : Anti-Wnt7b antibody (ab155313) at 1/5000 dilution
Lane 1 : Wnt 2 - transfected HEK-293T whole cell extracts
Lane 2 : Wnt 2b - transfected HEK-293T whole cell extracts
Lane 3 : Wnt 3 - transfected HEK-293T whole cell extracts
Lane 4 : Wnt 4 - transfected HEK-293T whole cell extracts
Lane 5 : Wnt 5a - transfected HEK-293T whole cell extracts
Lane 6 : Wnt 7a - transfected HEK-293T whole cell extracts
Lane 7 : Wnt 7b - transfected HEK-293T whole cell extracts, including V5-tag
Lane 8 : Wnt 8b - transfected HEK-293T whole cell extracts
Lane 9 : Wnt 9a - transfected HEK-293T whole cell extracts
Lane 10 : Wnt 9b - transfected HEK-293T whole cell extracts
Lane 11 : Wnt 10b - transfected HEK-293T whole cell extracts
Lane 12 : Wnt 16 - transfected HEK-293T whole cell extracts
Lysates/proteins at 72 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 39 kDa
The V5 was used as positive control at 1/2500 dilution shown at the bottom panel.
ab155313 has been referenced in 1 publication.
- Vallon M et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). PubMed: 30304675