Key features and details
- Rabbit polyclonal to Wnt7b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Wnt7b antibody
See all Wnt7b primary antibodies
DescriptionRabbit polyclonal to Wnt7b
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
Recombinant fragment within Human Wnt7b (internal sequence). The exact sequence is proprietary.
Database link: P56706
- WB: Human Wnt7b-V5 transfected HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Tissue specificityModerately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
FormWnt7b is a secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid modified and are ligands for members of the frizzled family of receptors, which mediate cell-cell communication during development. Wnt7b is thought to modulate several important signaling pathways in the lung which results in the coordinated proliferation of adjacent epithelial and mesenchymal cells to stimulate organ growth.
- Protein Wnt-7b antibody
- Wingless related MMTV integration site 7B antibody
- Wingless type MMTV integration site family member 7B antibody
All lanes : Anti-Wnt7b antibody (ab227607) at 1/5000 dilution
Lane 1 : Untransfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Human Wnt7b-V5 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227607 has not yet been referenced specifically in any publications.