Anti-Wnt8a antibody (ab227611)
Key features and details
- Rabbit polyclonal to Wnt8a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Wnt8a antibody
See all Wnt8a primary antibodies -
Description
Rabbit polyclonal to Wnt8a -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human Wnt8a (internal sequence). The exact sequence is proprietary.
Database link: Q9H1J5 -
Positive control
- WB: Human Wnt8a-transfected HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
|
Notes |
---|
IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
-
Function
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus. -
Sequence similarities
Belongs to the Wnt family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 540199 Cow
- Entrez Gene: 7478 Human
- Omim: 606360 Human
- SwissProt: Q9H1J5 Human
- Unigene: 591274 Human
-
Alternative names
- Protein Wnt-8a antibody
- Protein Wnt-8d antibody
- wingless type MMTV integration site family, member 8A antibody
see all
Images
-
All lanes : Anti-Wnt8a antibody (ab227611) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Human Wnt8a-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
-
Paraffin-embedded human colon carcinoma tissue stained for Wnt8a with ab227611 at 1/250 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab227611 has not yet been referenced specifically in any publications.