Key features and details
- Rabbit polyclonal to Wnt8b
- Suitable for: WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-Wnt8b antibody
See all Wnt8b primary antibodies
DescriptionRabbit polyclonal to Wnt8b
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Zebrafish
Recombinant fragment corresponding to Zebrafish Wnt8b aa 24-358.
WSVNNFLMTGPKAYLIYSSSVAAGAQSGIEECKYQFAWDRWKCPERALQL STHSGLRSANRETAFFHAISSAGVMYTLTRNCSLGDFDNCGCDDTRNGQR GGQGWLWGGCSDNVGFGEVISKQFVDALETGQDARAAMNLHNNEVGRKAV KGTMQRTCKCHGVSGSCTTQTCWLQLPEFREVGNYLKEKYHRAVKVDLLR GAGNSAASRGAIAETFNSISRKELVHLEDSPDYCLENRTLGLPGTEGREC LRKGKNLSKWEKRTCKRLCGDCGLAVEERRAETVSSCNCKFHWCCAVKCE QCRKTVTKYYCVKRSKRVKNDNASRRKSYRLKKKH
Database link: P51029
- WB: Zebrafish tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 40 kDa).|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Tissue specificityExpression is restricted to the brain, and more specifically to the forebrain.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt-8b antibody
- Wingless related MMTV integration site 8b antibody
- Wingless type MMTV integration site family, member 8B antibody
All lanes : Anti-Wnt8b antibody (ab224607) at 1/500 dilution
Lane 1 : Zebrafish tissue lysate at 40 µg
Lane 2 : Zebrafish tissue lysate at 20 µg
Lane 3 : Zebrafish tissue lysate at 10 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224607 has not yet been referenced specifically in any publications.