Product nameAnti-Wnt8b antibody
See all Wnt8b primary antibodies
DescriptionRabbit polyclonal to Wnt8b
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Fetal brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66307 in the following tested applications.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Tissue specificityExpression is restricted to the brain, and more specifically to the forebrain.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt-8b antibody
- Wingless related MMTV integration site 8b antibody
- Wingless type MMTV integration site family, member 8B antibody
Anti-Wnt8b antibody (ab66307) at 1.25 µg/ml (5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Tian BY et al. Specific knockdown of WNT8b expression protects against phosphate-induced calcification in vascular smooth muscle cells by inhibiting the Wnt-ß-catenin signaling pathway. J Cell Physiol 234:3469-3477 (2019). Read more (PubMed: 30461014) »