Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt8b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: KCHGVSGSCT TQTCWLQLPE FREVGAHLKE KYHAALKVDL LQGAGNSAAG, corresponding to internal sequence amino acids 181-230 of Human Wnt8b

  • Positive control

    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

Images

  • Anti-Wnt8b antibody (ab66307) at 1.25 µg/ml (5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 39 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

References

This product has been referenced in:

  • Tian BY  et al. Specific knockdown of WNT8b expression protects against phosphate-induced calcification in vascular smooth muscle cells by inhibiting the Wnt-ß-catenin signaling pathway. J Cell Physiol 234:3469-3477 (2019). Read more (PubMed: 30461014) »
See 1 Publication for this product

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