Anti-Wnt9b antibody (ab106928)
Key features and details
- Goat polyclonal to Wnt9b
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt9b antibody
See all Wnt9b primary antibodies -
Description
Goat polyclonal to Wnt9b -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide:
C-KRGNKDLRARADA
, corresponding to internal sequence amino acids 180-192 of Human Wnt9b (NP_003387.1). -
Positive control
- Human Brain Cerebellum lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106928 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Relevance
Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region. -
Cellular localization
Secreted: extracellular space: extracellular matrix. -
Database links
- Entrez Gene: 7484 Human
- Entrez Gene: 22412 Mouse
- Entrez Gene: 303586 Rat
- Omim: 602864 Human
- SwissProt: O14905 Human
- SwissProt: O35468 Mouse
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Alternative names
- MGC124412 antibody
- Protein Wnt 9b precursor antibody
- Protein Wnt-14b antibody
see all
Images
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All lanes : Anti-Wnt9b antibody (ab106928) at 1 µg/ml
Lane 1 : mouse kidney tissue lysate
Lane 2 : mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Anti-Wnt9b antibody (ab106928) at 0.3 µg/ml + Human Brain Cerebellum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab106928 has not yet been referenced specifically in any publications.