Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt9b
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human Wnt9b (internal sequence). A 16 amino acid synthetic peptide from the internal region of Human Wnt9b (NP_003387.1).

  • Positive control

    • Human fetal kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
  • Cellular localization

    Secreted: extracellular space: extracellular matrix.
  • Database links

  • Alternative names

    • MGC124412 antibody
    • Protein Wnt 9b precursor antibody
    • Protein Wnt-14b antibody
    • Protein Wnt-9b antibody
    • Wingless type MMTV integration site 9B antibody
    • Wingless type MMTV integration site family member 15 antibody
    • Wingless type MMTV integration site family member 9B antibody
    • Wnt 14b antibody
    • Wnt-15 antibody
    • Wnt14b antibody
    • Wnt15 antibody
    • Wnt9b wingless type MMTV integration site family member 9B antibody
    see all

Images

  • Immunohistochemistry of formalin-fixed, paraffin-embedded Human fetal kidney tissue staining Wnt9b using ab151220 at 10 ug/ml.

References

This product has been referenced in:

  • Zhou D  et al. Tubule-Derived Wnts Are Required for Fibroblast Activation and Kidney Fibrosis. J Am Soc Nephrol 28:2322-2336 (2017). Read more (PubMed: 28336721) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Liver
Blocking step
3% BSA/5% serum in PBS buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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