Product nameWnt/Beta Catenin Signaling Pathway Antibody Sampler Panel
ab228526, Wnt/Beta Catenin Signaling Pathway Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 6 recombinant rabbit monoclonal antibodies against c-Myc, c-Jun, Cyclin D1, LEF1, c-Met, and TCF7 provided in 10 µl trial sizes.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex assays. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit ab32072 - Anti-c Myc antibody [Y69] 1 x 10µl ab32137 - Anti-c-Jun antibody [E254] 1 x 10µl ab134175 - Anti-Cyclin D1 antibody [EPR2241] 1 x 10µl ab137872 - Anti-LEF1 antibody [EPR2029Y] 1 x 10µl ab51067 - Anti-Met (c-Met) antibody [EP1454Y] 1 x 10µl ab134127 - Anti-TCF7 antibody [EPR2035] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
- Anti-Cyclin D1 antibody [EPR2241] - BSA and Azide free (ab156448)
- Anti-Met (c-Met) antibody [EP1454Y] - BSA and Azide free (ab157370)
- Anti-c-Myc antibody [Y69] - BSA and Azide free (ab168727)
- Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free (ab215999)
- Anti-c-Jun antibody [E254] - BSA and Azide free (ab218576)
ab51067 staining Met (c-Met) in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween 20 and blocked for 30 minutes at 22°C; antigen retrieval was by heat mediation in Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674). Samples were incubated with the primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue, labeling Cyclin D1 with unpurified ab134175 at 1/100 dilution.
Immunofluorescent staining of HeLa cells labelling TCF7 with ab134127 at 1/100.
Immunofluorescence staining of Jurkat cells with purified ab137872 at a working dilution of 1 in 500, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab137872 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
ab32137 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) (ab120618), by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.
The cells were incubated at 37°C for 4h in media containing different concentrations of ab120618 (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32137 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IHC image of ab32072 staining c-Myc in human esophagus formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32072, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the Secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228526 has been referenced in 1 publication.
- Li Y et al. miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/ß-catenin and EMT signal pathways. J Orthop Surg Res 16:71 (2021). PubMed: 33472642