Overview

  • Product name

    Anti-Worniu antibody [5A3AD2]
  • Description

    Rat monoclonal [5A3AD2] to Worniu
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, IHCmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    5A3AD2
  • Isotype

    IgG2a
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab196362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC 1/100 - 1/250.

Target

  • Database links

    • Alternative names

      • BG:DS03023.1 antibody
      • br35 antibody
      • CG4158 antibody
      • CG4158-PA antibody
      • D.m.Worniu antibody
      • Dmel\CG4158 antibody
      • Dmel_CG4158 antibody
      • l(2)35Da antibody
      • l(2)br35 antibody
      • l35Da antibody
      • lethal(2)35Da antibody
      • schnecke antibody
      • wor antibody
      • wor-PA antibody
      see all

    Images

    • Immunohistochemistry analysis of Worniu in neuroblast nuclei from Drosophila stage 11 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.  The Worniu stain used ab196362 at 4.7 µg/mL, overnight at 4°C. The secondary antibody used was Alexa555 Magenta conjugated goat anti-rat (at 10 µg/mL).

    References

    This product has been referenced in:

    • Hakes AE  et al. A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development 145:N/A (2018). Read more (PubMed: 30254066) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab196362.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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