Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab18729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 72 kDa.|
FunctionFunctions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger.
modificationsSumoylated by SUMO1 and SUMO2/3.
Cellular localizationNucleus. Colocalizes with WRN in granular structures in the nucleus.
- Information by UniProt
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
ab18729 (4µg/ml) staining of paraffin embedded Human Kidney following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining shows nuclear staining, stronger in DCT compared to PCT.
Anti-WRNIP1 antibody (ab18729) at 1 µg/ml + Jurkat Cell Lysate in RIPA Buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
ab18729 has not yet been referenced specifically in any publications.