Overview

  • Product name
  • Description
    Rabbit polyclonal to WRNIP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human WRNIP1 aa 107-120 conjugated to keyhole limpet haemocyanin.
    Sequence:

    SRESYDAPPTPSGAC

Properties

Applications

Our Abpromise guarantee covers the use of ab4731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notes
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.
    • Post-translational
      modifications
      Sumoylated by SUMO1 and SUMO2/3.
    • Cellular localization
      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WHIP antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    Images

    • Western blot using ab4731 on HEK 293 lysate at 1/200.

       ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 protein.  The main band could be blocked using the immunising peptide.

      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1 or post translational modification of the WRNIP1 p

    References

    This product has been referenced in:
    • Kanu N  et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). Read more (PubMed: 26549024) »
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . Read more (PubMed: 17888034) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Loading amount
    10 µg
    Treatment
    lentivirus transduced for RNAi
    Specification
    293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Mar 05 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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