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    wrnip1whip-antibody-ab4731.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other
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Anti-WRNIP1/WHIP antibody (ab4731)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

    Key features and details

    • Rabbit polyclonal to WRNIP1/WHIP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-WRNIP1/WHIP antibody
      See all WRNIP1/WHIP primary antibodies
    • Description

      Rabbit polyclonal to WRNIP1/WHIP
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human WRNIP1/WHIP aa 107-120 conjugated to keyhole limpet haemocyanin.
      Sequence:

      SRESYDAPPTPSGAC

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

       This product was previously labelled as WRNIP1

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • Other
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4731 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB (1)
    Notes
  • Application notes
    ELISA: 1/5000 - 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 17888034).
    WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the AAA ATPase family.
      Contains 1 UBZ-type zinc finger.
    • Post-translational
      modifications

      Sumoylated by SUMO1 and SUMO2/3.
    • Cellular localization

      Nucleus. Colocalizes with WRN in granular structures in the nucleus.
    • Target information above from: UniProt accession Q96S55 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 56897 Human
      • Entrez Gene: 78903 Mouse
      • Entrez Gene: 282835 Rat
      • Omim: 608196 Human
      • SwissProt: Q96S55 Human
      • SwissProt: Q91XU0 Mouse
      • SwissProt: Q8CG07 Rat
      • Unigene: 236828 Human
      • Unigene: 286680 Mouse
      • Unigene: 8783 Rat
      see all
    • Alternative names

      • ATPase WRNIP 1 antibody
      • ATPase WRNIP1 antibody
      • bA420G6.2 antibody
      • FLJ22526 antibody
      • Putative helicase RUVBL antibody
      • RP11 420G6.2 antibody
      • Werner helicasae interacting protein 1 antibody
      • Werner helicasae interacting protein antibody
      • Werner helicase interacting protein isoform 2 antibody
      • Werner helicase-interacting protein 1 antibody
      • WHIP antibody
      • WRIP1_HUMAN antibody
      • WRNIP 1 antibody
      • WRNIP antibody
      • Wrnip1 antibody
      see all

    Images

    • Western blot - Anti-WRNIP1/WHIP antibody (ab4731)
      Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

      Western blot using ab4731 on HEK 293 lysate at 1/200.

       ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein.  The main band could be blocked using the immunising peptide.

      Western blot using ab4731 on HEK 293 lysate at 1/200.

      ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab4731? Please let us know so that we can cite the reference in this datasheet.

    ab4731 has been referenced in 2 publications.

    • Kanu N  et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). PubMed: 26549024
    • Mano Y  et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . PubMed: 17888034

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-WRNIP1/WHIP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8%)
    Loading amount
    10 µg
    Treatment
    lentivirus transduced for RNAi
    Specification
    293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 05 2009

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