Anti-WRNIP1/WHIP antibody (ab4731)
Key features and details
- Rabbit polyclonal to WRNIP1/WHIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WRNIP1/WHIP antibody
See all WRNIP1/WHIP primary antibodies -
Description
Rabbit polyclonal to WRNIP1/WHIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 107-120 conjugated to keyhole limpet haemocyanin.
Sequence:SRESYDAPPTPSGAC
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General notes
This product was previously labelled as WRNIP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
| Notes |
|---|
ICC/IF: Use at an assay dependent dilution (PMID 17888034).
WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger. -
Post-translational
modificationsSumoylated by SUMO1 and SUMO2/3. -
Cellular localization
Nucleus. Colocalizes with WRN in granular structures in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56897 Human
- Entrez Gene: 78903 Mouse
- Entrez Gene: 282835 Rat
- Omim: 608196 Human
- SwissProt: Q96S55 Human
- SwissProt: Q91XU0 Mouse
- SwissProt: Q8CG07 Rat
- Unigene: 236828 Human
see all -
Alternative names
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
see all
Images
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Western blot - Anti-WRNIP1/WHIP antibody (ab4731)
Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein. The main band could be blocked using the immunising peptide.
Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab4731 has been referenced in 2 publications.
- Kanu N et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). PubMed: 26549024
- Mano Y et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . PubMed: 17888034