Anti-WRNIP1/WHIP antibody (ab4731)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-WRNIP1/WHIP antibody
See all WRNIP1/WHIP primary antibodies -
Description
Rabbit polyclonal to WRNIP1/WHIP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 107-120 conjugated to keyhole limpet haemocyanin.
Sequence:SRESYDAPPTPSGAC
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General notes
This product was previously labelled as WRNIP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| ELISA |
ICC/IF: Use at an assay dependent dilution (PMID 17888034).
WB: 1/200. Detects a band of approximately 97 kDa (predicted molecular weight: 72 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger. -
Post-translational
modificationsSumoylated by SUMO1 and SUMO2/3. -
Cellular localization
Nucleus. Colocalizes with WRN in granular structures in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56897 Human
- Entrez Gene: 78903 Mouse
- Entrez Gene: 282835 Rat
- Omim: 608196 Human
- SwissProt: Q96S55 Human
- SwissProt: Q91XU0 Mouse
- SwissProt: Q8CG07 Rat
- Unigene: 236828 Human
see all -
Alternative names
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
see all
Images
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Western blot - Anti-WRNIP1/WHIP antibody (ab4731)Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein. The main band could be blocked using the immunising peptide.
Western blot using ab4731 on HEK 293 lysate at 1/200.
~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .
References
This product has been referenced in:
- Kanu N et al. RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress. Oncogene 35:4009-19 (2016). Read more (PubMed: 26549024) »
- Mano Y et al. Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer. Cancer Sci 98:1902-13 (2007). ICC/IF ; Human . Read more (PubMed: 17888034) »
