Overview

  • Product name

  • Description

    Rabbit polyclonal to WRNIP1/WHIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human WRNIP1/WHIP aa 400-450.
    Database link: NP_064520.2

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as WRNIP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab99316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the AAA ATPase family.
    Contains 1 UBZ-type zinc finger.
  • Post-translational
    modifications

    Sumoylated by SUMO1 and SUMO2/3.
  • Cellular localization

    Nucleus. Colocalizes with WRN in granular structures in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATPase WRNIP 1 antibody
    • ATPase WRNIP1 antibody
    • bA420G6.2 antibody
    • FLJ22526 antibody
    • Putative helicase RUVBL antibody
    • RP11 420G6.2 antibody
    • Werner helicasae interacting protein 1 antibody
    • Werner helicasae interacting protein antibody
    • Werner helicase interacting protein isoform 2 antibody
    • Werner helicase-interacting protein 1 antibody
    • WHIP antibody
    • WRIP1_HUMAN antibody
    • WRNIP 1 antibody
    • WRNIP antibody
    • Wrnip1 antibody
    see all

Images

  • Anti-WRNIP1/WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg

    Secondary
    IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WRNIP1/WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-WRNIP1/WHIP antibody (ab99316) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 30 seconds
  • Detection of WRNIP1/WHIP by Western Blot of Immunprecipitate.
    ab99316, at 1 µg/ml, staining WRNIP1/WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab99316 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (T cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
80 µg
Specification
T cell
Blocking step
LI-COR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 07 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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