Key features and details
- Rabbit polyclonal to WRNIP1/WHIP
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-WRNIP1/WHIP antibody
See all WRNIP1/WHIP primary antibodies
DescriptionRabbit polyclonal to WRNIP1/WHIP
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human WRNIP1/WHIP aa 400-450.
Database link: NP_064520.2
- HeLa whole cell lysate
This product was previously labelled as WRNIP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99316 was affinity purified using an epitope specific to WRNIP1/WHIP immobilized on solid support
Our Abpromise guarantee covers the use of ab99316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFunctions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the AAA ATPase family.
Contains 1 UBZ-type zinc finger.
modificationsSumoylated by SUMO1 and SUMO2/3.
Cellular localizationNucleus. Colocalizes with WRN in granular structures in the nucleus.
- Information by UniProt
- ATPase WRNIP 1 antibody
- ATPase WRNIP1 antibody
- bA420G6.2 antibody
Anti-WRNIP1/WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µg
IRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WRNIP1/WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-WRNIP1/WHIP antibody (ab99316) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 30 seconds
Detection of WRNIP1/WHIP by Western Blot of Immunprecipitate.
ab99316, at 1 µg/ml, staining WRNIP1/WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab99316 has not yet been referenced specifically in any publications.