Anti-WSB1 antibody (ab235090)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-WSB1 antibody
See all WSB1 primary antibodies -
Description
Rabbit polyclonal to WSB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human WSB1 aa 201-421.
Sequence:KDDGNMMKVLRGHQNWVYSCAFSPDSSMLCSVGASKAVFLWNMDKYTMIR KLEGHHHDVVACDFSPDGALLATASYDTRVYIWDPHNGDILMEFGHLFPP PTPIFAGGANDRWVRSVSFSHDGLHVASLADDKMVRFWRIDEDYPVQVAP LSNGLCCAFSTDGSVLAAGTHDGSVYFWATPRQVPSLQHLCRMSIRRVMP TQEVQELPIPSKLLEFLSYRI
Database link: Q9Y6I7 -
Positive control
- WB: Rat heart tissue lysate. IHC-P: Human appendix and kidney tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab235090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| WB | 1/500 - 1/5000. Predicted molecular weight: 47 kDa. |
Target
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Relevance
WSB1 is a member of the WD-protein subfamily. It shares a high sequence identity to mouse and chick proteins. It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus. It is a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It recognizes type II iodothyronine deiodinase/DIO2. There are two named isoforms. -
Cellular localization
Intracellular -
Database links
- Entrez Gene: 26118 Human
- Entrez Gene: 78889 Mouse
- Entrez Gene: 303336 Rat
- Omim: 610091 Human
- SwissProt: Q9Y6I7 Human
- SwissProt: O54927 Mouse
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Alternative names
- SOCS box containing WD protein SWiP 1 antibody
- SWIP1 antibody
- WD repeat and SOCS box containing 1 antibody
see all
Images
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Western blot - Anti-WSB1 antibody (ab235090)Anti-WSB1 antibody (ab235090) at 1/500 dilution + Rat brain tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSB1 antibody (ab235090)Paraffin-embedded human appendix tissue stained for WSB1 using ab235090 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSB1 antibody (ab235090)Paraffin-embedded human kidney tissue stained for WSB1 using ab235090 at 1/100 dilution in immunohistochemical analysis.
References
ab235090 has not yet been referenced specifically in any publications.
