WST-1 Assay Kit (Cell Proliferation) (ab65473)

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Overview

  • Product name

    WST-1 Assay Kit (Cell Proliferation)
    See all Cell viability/proliferation kits

  • Detection method

    Colorimetric

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Quantitative

  • Assay time

    4h 00m

  • Product overview

    WST-1 Cell Proliferation Assay Kit (ab65473) provides by far the easiest and most sensitive means for performing a quantitative cell proliferation assay, cell viability assay, or cytotoxicity assay in mammalian cells. It is also available in a ready-to-use reagent format: ab155902.


    The WST-1 assay protocol is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by microplate reader by measuring the absorbance of the dye solution at 440 nm.


    The WST-1 assay is just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.


    The WST-1 assay protocol is very simple:
    - add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
    - shake the plate to mix the contents
    - analyze the amount of formazan dye produced by measuring the absorbance at 440 nm

  • Notes

    This kit was previously called Quick Cell Proliferation Assay Kit.

    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

  • Platform

    Microplate reader

Properties

References (14)

Publishing research using ab65473? Please let us know so that we can cite the reference in this datasheet.

ab65473 has been referenced in 14 publications.

  • Dehghan-Baniani D  et al. Injectable in situ forming kartogenin-loaded chitosan hydrogel with tunable rheological properties for cartilage tissue engineering. Colloids Surf B Biointerfaces 192:111059 (2020). PubMed: 32380404
  • Langford-Smith AWW  et al. Diabetic endothelial colony forming cells have the potential for restoration with glycomimetics. Sci Rep 9:2309 (2019). PubMed: 30783159
  • Arodin Selenius L  et al. The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells. Antioxidants (Basel) 8:N/A (2019). PubMed: 31091728
  • Gulyaeva O  et al. Sox9-Meis1 Inactivation Is Required for Adipogenesis, Advancing Pref-1+ to PDGFRa+ Cells. Cell Rep 25:1002-1017.e4 (2018). PubMed: 30355480
  • Li LQ  et al. Sensitization of Gastric Cancer Cells to 5-FU by MicroRNA-204 Through Targeting the TGFBR2-Mediated Epithelial to Mesenchymal Transition. Cell Physiol Biochem 47:1533-1545 (2018). PubMed: 29940566
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

ab65473
What is the reference wavelength used for? At 650 nm?

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Answer

Thank you for your telephone call this morning.

The absorption measured at the reference wavelength value is subtracted from each reading. This is to normalize the readings against a non changing value. One wavelength reports the change in absorption from enzyme action. The other (in this case at 650 nm) should remain the same since it is set at a value unrelated to expected absorption values.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Since this kit requires measurement of absorbance, is there a requirement of standard curve? If so, what do I use to generate the curve?

If standard curve is not required, is there something I can reference my readings to (e.g positive control)?
Much appreciate your time

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Answer

The assay kit has not been designed to use with a standard curve as such. You can indeed reference your readings if you need to. Any cell type that is actively dividing in culture can be used as a positive control. Any cell type that is treated with a known cell proliferation inducer (growth factors) can also be used.

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Question

I would like to know what the difference is between proliferation kit 65473 and 65475. Is 65475 a newer version of 65473 or a more sensitive version?

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Answer


The primary difference between the quick cell proliferiation assay (ab65473) and quick cell plus proliferation assay (ab65475) is that the ab65475 is more sensitive and more stable.

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Question

Dear Sir/Madame,
I would like to have more information about your two products: "Quick Cell Proliferation Assay Kit (ab65473)" and "Quick Cell Proliferation Assay Kit II (ab65475)"
In particular I would ask if the serum in the culture medium could interferes with the analysis.
Kindly Regards

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Answer

Thank you for contacting us and your interest in our products.

The Quick Cell Proliferation Assay Kit (ab65473) and Quick Cell Proliferation Assay Kit II (ab65475) should not be affected by serum in your culture medium. Any differences from the different media which may be used will be accounted for by the control of the same sample and subtracting the readings of such controls from the sample readings. This procedure for this is described in the protocol of both the kits.

Hope this information has been useful for you. Please let me know if you have any other questions.

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Question

Hello, I would like to order a "Quick Cell Proliferation Assay Kit" but I have a problem with the volume in which I have to cultivate the cells. It's written in the online protocol that we have to grow the cells on a 96 well-plate but it's not possible in my case. Can I still cultivate the cells in my usual plates and then pipette the medium to a 96 well-plate to quantify the number of cells? Or maybe have you an other suggestion. Thank you very much for your help. regards,

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Answer

Thank you for your inquiry. I have asked the laboratory with your question. Indeed, the laboratory recommend just that if the cells are cultured in different volume of culture medium, increase or decrease the amount of WST-1/ECS solution correspondingly. There is no problem therefore to cultivate the cells in another format, you will just need to adjust the volumes correspondingly. Then transfer 100 ul to a 96-well plate to read. I hope this information is helpful. Please do not hesitate to contact us again should you have any other question.

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Question

Difference between ab65473,ab65474

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Answer

ab65474 - enables to perform 2500 assays instead of 500 (like ab65473) and includes a premade stop solution (unlike ab65473). Also the kits include different tetrazolium salts (WST or WST-1 reagent). More details cannot be given out due to proprietary reasons.

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