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  1. Link

    wst-1-assay-kit-cell-proliferation-ab65475.pdf

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WST-1 Assay Kit (Cell Proliferation) (ab65475)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (6)References (9)

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Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 4 hr
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    WST-1 Assay Kit (Cell Proliferation)
    See all Cell viability/proliferation kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay time

    4h 00m
  • Product overview

    WST-1 Assay Kit ab65475 is an alternative version of ab65473, our most popular WST-1 Assay Kit (which is also supplied as a ready-to-use reagent ab155902). This kit uses a different WST-1 analog, and also contains a stop solution.


    The WST-1 assay provides by far the easiest and most sensitive means for performing a quantitative cell proliferation assay, cell viability assay, or cytotoxicity assay in mammalian cells.


    The WST-1 assay protocol is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by microplate reader by measuring the absorbance of the dye solution at 440 nm.


    The WST-1 assay protocol is very simple:
    - add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
    - shake the plate to mix the contents
    - analyze the amount of formazan dye produced by measuring the absorbance at 440 nm
    This kit contains an optional stop solution to stop the production of formazan dye.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K302 Quick Cell Proliferation colorimetric Assay Kit Plus. K302-2500 is the same size as the 2500 test size of ab65475.

    The WST-1 assay is add-and-read, and requires no washing, harvesting, or solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.

    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests 2500 tests
    Electro Coupling Solution (ECS) 1 x 5ml 1 x 25ml
    Stop Solution 1 x 5ml 1 x 25ml
    WST Reagent (lyophilized) 1 vial 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Cell Viability and Senescence Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • proliferation and senescence
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Associated products

  • Assay kits

    • WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)
    • WST-1 Assay Kit (Cell Proliferation) (ab65473)

Images

  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
    Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II

    Mitochondrial dehydrogenases checked in various cell concentrations at different incubation times (2h WST for each condition)

  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
    Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II

    HL60 cell proliferation measured after 24h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations

  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
    Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II

    HL60 cell proliferation measured after 48h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations

  • Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II
    Cellular activity - ab65475 Quick Cell Proliferation Assay Kit II

    HL60 cell proliferation measured after 72h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab65475? Please let us know so that we can cite the reference in this datasheet.

ab65475 has been referenced in 9 publications.

  • Omote N  et al. Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation state. Physiol Rep 9:e14727 (2021). PubMed: 33527707
  • Chaudhuri P  et al. P110a and P110d catalytic subunits of PI3 kinase regulate lysophosphatidylcholine-induced TRPC6 externalization. Am J Physiol Cell Physiol 320:C731-C741 (2021). PubMed: 33625929
  • Villa-Bellosta R Dietary magnesium supplementation improves lifespan in a mouse model of progeria. EMBO Mol Med 12:e12423 (2020). PubMed: 32875720
  • Ali A  et al. LIN28-let-7 axis regulates genes in immortalized human trophoblast cells by targeting the ARID3B-complex. FASEB J 33:12348-12363 (2019). PubMed: 31415216
  • Hyndman KA  et al. Dynamic changes in histone deacetylases following kidney ischemia-reperfusion injury are critical for promoting proximal tubule proliferation. Am J Physiol Renal Physiol 316:F875-F888 (2019). PubMed: 30810062
  • Tan D  et al. Inhibition of RhoA-Subfamily GTPases Suppresses Schwann Cell Proliferation Through Regulating AKT Pathway Rather Than ROCK Pathway. Front Cell Neurosci 12:437 (2018). PubMed: 30515082
  • Khedr MEMS  et al. Vasoactive intestinal peptide induces proliferation of human hepatocytes. Cell Prolif 51:e12482 (2018). PubMed: 30028555
  • Racioppi L  et al. Calcium/calmodulin-dependent kinase kinase 2 regulates hematopoietic stem and progenitor cell regeneration. Cell Death Dis 8:e3076 (2017). PubMed: 28981105
  • Patsenker E  et al. 4-O'-methylhonokiol protects from alcohol/carbon tetrachloride-induced liver injury in mice. J Mol Med (Berl) 95:1077-1089 (2017). PubMed: 28689299

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

I would like to know what the difference is between proliferation kit 65473 and 65475. Is 65475 a newer version of 65473 or a more sensitive version?

Read More

Abcam community

Verified customer

Asked on Aug 16 2013

Answer


The primary difference between the quick cell proliferiation assay (ab65473) and quick cell plus proliferation assay (ab65475) is that the ab65475 is more sensitive and more stable.

Read More

Sybille Rex

Abcam Scientific Support

Answered on Aug 16 2013

Question

Dear Sir/Madame,
I would like to have more information about your two products: "Quick Cell Proliferation Assay Kit (ab65473)" and "Quick Cell Proliferation Assay Kit II (ab65475)"
In particular I would ask if the serum in the culture medium could interferes with the analysis.
Kindly Regards

Read More

Abcam community

Verified customer

Asked on Jan 09 2013

Answer

Thank you for contacting us and your interest in our products.

The Quick Cell Proliferation Assay Kit (ab65473) and Quick Cell Proliferation Assay Kit II (ab65475) should not be affected by serum in your culture medium. Any differences from the different media which may be used will be accounted for by the control of the same sample and subtracting the readings of such controls from the sample readings. This procedure for this is described in the protocol of both the kits.

Hope this information has been useful for you. Please let me know if you have any other questions.

Read More

Abcam Scientific Support

Answered on Jan 09 2013

Question

Inquiry: Hello, We are currently using a Quick cell proliferation assay kit II (#ab65475) in our lab and I would like to know the minimum sensitivity (ie OD) the kit can detect. Below which OD value can we consider the reading as non-relevant (even if we substract an initial background)? Regards,

Read More

Abcam community

Verified customer

Asked on Sep 19 2012

Answer

Thank you for your enquiry and your interest in our products.

As I understand you are looking for an absolute cut-off value for the OD. This is a comparative assay in which you can let the colour develop for an extended period of time to look at minor differences between various samples.

I would advise to perform the statistical significance analysis on the differences you get form the use of this kit. You need to deem the control sample’s OD at 100% cellular proliferation or 0% cell toxicity and go from there in their comparative analysis.

I hope this information will be useful for you.

Read More

Abcam Scientific Support

Answered on Sep 19 2012

Question

- The absorbance reading still going up even after 5 hours of incubation.

Read More

Abcam community

Verified customer

Asked on Jun 08 2012

Answer

Thank you for contacting us.
I have now received an answer from lab. They say the colour might develop with longer incubation of the samples. However, the machine can detect the absorbance only within a certain limit. Keeping that in mind, we recommend repeated reads and termination of the reaction by adding the stop buffer whenever the desired reading is achieved. The 0.5-4 hrs is just the expected time range within which this is expected to happen.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Jun 08 2012

Question

The directions in the datasheet note that "Phenol Red in culture medium does not significantly interfere with the assay." But how will phenol red effect the assay and to what degree?

Read More

Abcam community

Verified customer

Asked on Oct 25 2011

Answer

The phenol red from the sample will be diluted in the reaction buffer to a degree that it will not interfere with the assay.

Read More

Abcam Scientific Support

Answered on Oct 25 2011

Question

Also, I am starting a new lab Univ of Missouri, Columbia. What sort of new lab start up promotions are available?

Read More

Abcam community

Verified customer

Asked on Oct 24 2011

Answer

Thank you for your enquiry. Currently our new lab promotion allows 20% off ALL orders plus FREE shipping on all orders for 3 months. The requirements to be considered a “New Lab” are the following: The lab is created by a scientist starting a completely new lab. Scientist moving existing lab to a new location. Scientist is a first time grant recipient. If one of these describes you currently, please email newlabdiscount@abcam.com

Read More

Abcam Scientific Support

Answered on Oct 24 2011

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