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- The absorbance reading still going up even after 5 hours of incubation.
Asked on Jun 08 2012
Thank you for contacting us.
I have now received an answer from lab. They say the colour might develop with longer incubation of the samples. However, the machine can detect the absorbance only within a certain limit. Keeping that in mind, we recommend repeated reads and termination of the reaction by adding the stop buffer whenever the desired reading is achieved. The 0.5-4 hrs is just the expected time range within which this is expected to happen.
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Answered on Jun 08 2012