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Inquiry: Hello, We are currently using a Quick cell proliferation assay kit II (#ab65475) in our lab and I would like to know the minimum sensitivity (ie OD) the kit can detect. Below which OD value can we consider the reading as non-relevant (even if we substract an initial background)? Regards,
Asked on Sep 19 2012
Thank you for your enquiry and your interest in our products.
As I understand you are looking for an absolute cut-off value for the OD. This is a comparative assay in which you can let the colour develop for an extended period of time to look at minor differences between various samples.
I would advise to perform the statistical significance analysis on the differences you get form the use of this kit. You need to deem the control sample’s OD at 100% cellular proliferation or 0% cell toxicity and go from there in their comparative analysis.
I hope this information will be useful for you.
Answered on Sep 19 2012