Overview

  • Product name
    WST-1 Assay Reagent - Cell Proliferation (ready to use)
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    0h 30m
  • Product overview

    WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells.


    The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. The larger the number of viable cells, the higher the activity of the mitochondrial dehydrogenases, and in turn the greater the amount of formazan dye formed.


    The WST-1 assay protocol is very simple:
    - add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
    - shake the plate to mix the contents
    - analyze the amount of formazan dye produced by measuring the absorbance at 440 nm


    We also offer complete WST-1 assay kits: WST-1 assay kit ab65473 which is the full kit version of this reagent,  and WST-1 assay kit ab65475 which uses an alternative WST-1 analog.

  • Notes

    WST-1 assays are non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

    Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.

Properties

Protocols

References

This product has been referenced in:
  • Zaatiti H  et al. Tumorigenic proteins upregulated in the MYCN-amplified IMR-32 human neuroblastoma cells promote proliferation and migration. Int J Oncol 52:787-803 (2018). Read more (PubMed: 29328367) »
  • Senathilake KS  et al. Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro. Exp Parasitol 177:13-21 (2017). Human . Read more (PubMed: 28351683) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

cell proliferation in mice splenocytes

Below average Excellent 5/5 (Ease of Use)
Abreviews
- cell concentration should be optimized
- reagent volume should be adapted
- Incubation time should be optimized

Abcam user community

Verified customer

Submitted Oct 26 2017

Sensitive and reliable reagent.

Good Excellent 5/5 (Ease of Use)
Abreviews
The starting colour of the reagent was different to the pink starting colour I have seen with WST-1 reagent from a different company. However, this did not seem to affect the sensitivity or reliability of the assay and I obtained good, reproducible results.

Abcam user community

Verified customer

Submitted Jan 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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