WST-1 Assay Reagent - Cell Proliferation (ready-to-use) (ab155902) provides a simple and accurate method to measure cell proliferation in mammalian cells.
The WST-1 assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm.
WST-1 assays are non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
We also offer complete WST-1 assay kits: WST-1 assay kit ab65473 and WST-1 assay kit ab65475.
Zaatiti H et al. Tumorigenic proteins upregulated in the MYCN-amplified IMR-32 human neuroblastoma cells promote proliferation and migration. Int J Oncol52:787-803 (2018).
Read more (PubMed: 29328367) »
Senathilake KS et al. Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro. Exp Parasitol177:13-21 (2017).
Read more (PubMed: 28351683) »