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WST-1 Cell Proliferation Assay Kit II provides by far the easiest and most sensitive means for quantifying cell proliferation and viability in mammalian cells.
WST-1 assays are based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm.
The WST-1 assay is just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
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This kit was previously called Quick Cell Proliferation Assay Kit II.
|Components||500 tests||2500 tests|
|Electro Coupling Solution (ECS)||1 x 5ml||1 x 25ml|
|Stop Solution||1 x 5ml||1 x 25ml|
|WST Reagent (lyophilized)||1 vial||1 vial|
Mitochondrial dehydrogenases checked in various cell concentrations at different incubation times (2h WST for each condition)
HL60 cell proliferation measured after 24h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
HL60 cell proliferation measured after 48h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
HL60 cell proliferation measured after 72h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
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