• Product name
  • Description
    Rabbit polyclonal to WSTF
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: APLLGRKPFPLVKPLPGEE, corresponding to amino acids 2-20 of Human WSTF conjugated to KLH by a C terminal Cysteine residue linker.

  • Positive control
    • Extracts of MCF-7 breast cancer cell line.



Our Abpromise guarantee covers the use of ab50850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 171 kDa).

Staining of the WSTF band in immunoblotting is specifically inhibited by the immunizing peptide.

IP Use at an assay dependent concentration.

5-10 µg immunoprecipitates WSTF from lysates of HEK293-T cells.


  • Function
    Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association.
  • Tissue specificity
    Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
  • Involvement in disease
    Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities
    Belongs to the WAL family. BAZ1B subfamily.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 WAC domain.
  • Developmental stage
    Expressed at equal levels in 19-23 weeks old fetal tissues.
  • Domain
    The N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
    The bromo domain mediates the specific interaction with acetylated histones.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.
  • Information by UniProt
  • Database links
  • Alternative names
    • baz1b antibody
    • BAZ1B_HUMAN antibody
    • Bromodomain adjacent to zinc finger domain protein 1B antibody
    • hWALP 2 antibody
    • hWALP-2 antibody
    • hWALP2 antibody
    • transcription factor WSTF antibody
    • Tyrosine-protein kinase BAZ1B antibody
    • WALP-2 antibody
    • WALP2 antibody
    • WBRS 9 antibody
    • WBRS-9 antibody
    • WBRS9 antibody
    • WBSC 10 antibody
    • WBSC-10 antibody
    • WBSC10 antibody
    • WBSCR10 antibody
    • WBSCR9 antibody
    • Williams Beuren syndrome chromosome region 9 protein antibody
    • Williams syndrome transcription factor antibody
    • Williams-Beuren syndrome chromosomal region 10 protein antibody
    • Williams-Beuren syndrome chromosomal region 9 protein antibody
    • WSTF antibody
    see all


  • All lanes : Anti-WSTF antibody (ab50850) at 0.5 µg/ml

    Lane 1 : HEK-293T extract
    Lane 2 : MCF7 extract
    Lane 3 : Jurkat extract
    Lane 4 : C2 extract
    Lane 5 : 3T3 extract
    Lane 6 : Mouse brain extract
    Lane 7 : PC12 extract
    Lane 8 : AT3B-1 extract
    Lane 9 : RAT1 extract

    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size: 171 kDa
    Observed band size: 177 kDa (why is the actual band size different from the predicted?)

  • Lane 1 : Anti-WSTF antibody (ab50850) at 2 µg/ml
    Lanes 2 & 4-5 : Anti-WSTF antibody (ab50850) at 1 µg/ml
    Lane 3 : Anti-WSTF antibody (ab50850) at 0.5 µg/ml

    Lanes 1-3 : Mcf7 cell lysate
    Lane 4 : Mcf7 cell lysate with Non-relevant peptide at 10 µg/ml
    Lane 5 : Mcf7 cell lysate with WSTF immunizing peptide at 10 µg/ml

    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size: 171 kDa
    Observed band size: 210 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Almuzzaini B  et al. In ß-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects. FASEB J 30:2860-73 (2016). Read more (PubMed: 27127100) »
  • Geiger R  et al. L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor Activity. Cell 167:829-842.e13 (2016). Human . Read more (PubMed: 27745970) »

See all 6 Publications for this product

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